General information
The JHU DNA Analysis Facility is a division of the Genetic Resources Core Facility (GRCF) at a Johns Hopkins University with the aim to aid researchers performing studies in molecular biology and genetics. Additionally, the GRCF sponsors various educational programs, product seminars and an annual symposium.
Sequencing instruments used
Applied Biosystems 3730xl
Prices
Single pre-mixed template & primer
$7 per sample
95 samples in a 96 well plate
$6.50 per sample | $617.50 per plate
BAC protocol reactions
Used on BACs, other large templates or very GC rich templates | $25
Same day sequencing service
$20 per sample, minimum of 5 samples
Sample requirements
Samples should be provided pre-mixed in 1.5 ml tubes. In each tube mix 6 ul of primer and 6 ul of template. Use only one primer per tube.
Plasmids
200 ng/µl
PCR products
3 ng/µl/100 bp
BAC/PAC/YAC
Contact JHU for instructions
Primers
10 pmol/µl
Common universal primers are provided free
Sample turn around time
24 hours
International orders
Yes
GLP DNA sequencing
No
Other DNA sequencing related services offered
- Pyrosequencing
- Next generation sequencing
- Real time PCR
- SNP analysis
Contact Information
Johns Hopkins University
Blalock 1005
600 N. Wolfe St.
Baltimore, MD 21287
Phone: 410-955-2836
Hours: Monday - Friday 7:00 AM - 4:30 PM
Comments
Good quality core facility offering a range of sequencing services at reasonable prices. Very friendly.
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