General Information
Established in 1993, Baseclear specialize in offering a range of DNA sequencing and related services such as genotyping, Oligonucleotide synthesis and DNA purification. BaseClear also offer a range of molecular biology kits and equipment. Based in BioSciencePark of Leiden, The Netherlands.
Sequencing Instruments Used
Not stated but ABI 3730xls implied
Sequencing Service Prices
Full DNA Sequencing Service
Includes template concentration measurement, manual editing, free re-run of failed sequencing reactions, and phred base calling. Template can be supplied as either purified or unpurified PCR or plasmids.
Short Reads (~550 bases) | Contact BaseClear for pricing.
Long Reads (~800 bases) | Contact BaseClear for pricing.
24 Hour DNA Sequence Service
Samples received before 10 am are returned to customers within 24 hours. Includes measurement of the template concentration and phred base calling. No manual trace editing or free re-run of failed sequencing reactions. Only purified DNA templates are sequenced.
Short Reads (~550 bases) | Contact Base Clear for current price.
Long Reads (~800 bases) | Contact Base Clear for current price.
Quick Shot Sequencing service
Lowest cost service. Requires customer delivery premixed template and primer. Short Quick Shot Short Reads (~550 bases) | Contact Base Clear for current price.
Quick Shot Long Reads (~800 bases) | Contact Base Clear for current price.
Difficult Template Sequencing
Includes high G+C templates, BAC sequencing, oligonucleotide sequencing, direct genomic DNA sequencing, RNAi constructs and other templates with strong secondary structure. Template concentration is measured and failed samples re-run as per the full service.
Contact for Base Clear for pricing.
High Volume DNA Sequencing Service
Minimum of 95 reaction. Samples must be delivered in 96- or 384-well trays. Sequencing performed under either Full Service or 24 hour conditions
Contact for BaseClear for pricing.
Genome Project Sequencing
Full service genome or project sequencing including gene library construction, plasmid purification, primer design and/or synthesis, plus assembly and editing.
Contact for BaseClear for pricing.
Purified Template Sample Requirements
Plasmids - Quick Shot Sequencing
750 ng + 25 pmol primer in 20 µl total volume
PCR products
15 ng per 100 bp + 25 pmol of primer in 20 µl total volume
Other
All purified templates must be delivered in water of 10mM Tris (pH 8). Samples should be free of EDTA and/or ethanol.
Sample turn around time
24 - 48 hours depending on service
International orders
Yes
GLP DNA sequencing
Unknown
Other DNA sequencing related services offered
- Genome sequencing
- DNA/RNA purification
- DNA synthesis
- Gene library construction
- Molecular typing of microorganisms
- Genotyping and SNP analysis
- Primer walking sequencing
- Oligonucleotide sequencing
Contact Information
BaseClear - Street Address
Einsteinweg 5
2333 CC LEIDEN
The Netherlands
BaseClear - Mail Address
P.O. Box 1336
2302 BH LEIDEN
The Netherlands
ph.+31 (0)71 523 3917
fax. +31 (0)71 523 5594
email. info@baseclear.com
Comments
BaseClear have are no prices on their website. Baseclear appear quite large (25 technical staff) and professional. Their full service sequencing reaction offers a range of features (template concentration checking, re-run of failed reactions) that would be helpful for people inexperienced with DNA sequencing. A nice clean website design too which is nice to see.
Return to the main DNA sequencing service review page.