General information
Established in 1999, VBC-Biotech Services GmbH offers a range of DNA sequencing related services and products. As of 2003 VBC-Biotech employees 32 staff. VBC-Biotech are based in Vienna, Austria.
Sequencing Instruments Used
Sequencing Prices
Ready to go (electrophoresis only)
€9.00 per reaction
Users need to provide the sample names in an excel spreadsheet (rows A1-H12).
Single-Read Sequencing (Automatic)
Up to 400bp | €16.50 per reaction
Up to 800bp | €28.00 per reaction
Single-Read Sequencing (Manual editing and troubleshooting assistance)
Up to 400bp | €23.80 per reaction
Up to 800bp | €35.50 per reaction
Failed reactions
€11.00 per sample
Primer walking sequencing (max 5kB templates)
€0.15 per bp single DNA strand
€0.30 per bp both DNA strands
Walking service includes: Primer design and synthesis, manually editing of sequences, sequence assembly, sequence data in text and scf format, and personal assistance (e.g. Troubleshooting).
Additional Services
PCR product purification
1-9 samples | €13,00 EUR
10-49 samples | €11.50 EUR
> 50 samples | €9.00 EUR
Primer design
€9.00
Sequencing primer
€10.50
Sequence assembling
€8.00
Sample Requirements
Plasmids
min 80 ng/µl | 0.3 to 0.5 µg total
PCR products
min 14 fmol/µl | 80 - 100 fmol total
Primers
10 pmol/µl | 10 - 20 µl minimum
Common universal primers are provided free
Primer Walking templates
ssDNA | 2 µg/kb
dsDNA | 5 µg/kb
Sample turn around time
24 hours
International orders
Yes
GLP DNA sequencing
Unknown
Other DNA sequencing related services offered
- DNA/RNA-Oligonucleotide Synthesis
- Affymetrix GeneChip Services
- Custom Oligonucleotide Microarrays
- PCR Product Purification
- Primer Design
- Sequence Assembly
- tRFLP / RFLP Analysis
- AFLP Analysis
- Microsatellite and STR Analysis
Contact Information
VBC-Biotech Services GmbH
Rennweg 95B
1030 Wien
Austria
Tel: +43 1 796 65 72-60
Fax: +43 1 796 65 72-321
email. sequencing@vbc-biotech.com
Comments
An expensive service but high quality service. This includes verification of clones, cloning sites, analysis of cDNAs (ESTs), identification of genes and verification of PCR products, a DNA quality check, supply of sequence in .ab1, .scf and .txt format, and if required repetition of the sequencing reaction under modified conditions. Customers DNA and primers are stored for 3 month. Offer a very fast turn around time which is nice.
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