The latest version of Auto PeakTrace RP (5.50) has been released. These are the changes
- Improved fuse basecalling option.
- Improved basecalling through large dye peaks.
- Default options changed to improve basic usage
- .seq file output: abi format
- required good base improvement: -20
- q average trim: Q12 average in 20 base window
- n base threshold: 5
- skip short traces: yes
- A short trace is now considered to be a trace with fewer than 500 bases.
- Improved support for ET terminator traces.
- Improved support for ABI 3500 BigDye 1.1 traces.
- New error code 36 added – Input and output paths are the same.
- New error code 38 added – Skipped short trace.
- Good quality threshold and N base threshold now have maximum values of 99.
- The usual bug fixes and tweaks.
The update is available from the Nucleics downloads page and the automatic updater will be be live later this week.