General Information
The DNA Sequencing Core is one of the facilities of the Cardiff University, School of Biosciences. The Cardiff School of Biosciences is one of the largest bioscience departments in the UK. Supported by state-of-the-art facilities, its research spans the Life Sciences from whole (eco) systems to molecular biology.
DNA Sequencing Instruments Used
- Applied Biosystems (Model not mentioned)
DNA Sequencing Prices
Not mentioned
DNA Template Requirements
Plasmids
- 50-100ng/µl | 15µl
PCR products
-
150-300bp: 2ng/µl | 15µl
-
300-1000bp: 5ng/µl | 15µl
-
>1000bp: 10ng/µl | 15µl
Primers
2 pmol/µl |15µl
Other information
Samples should be clean and eluted in water and submitted in a 1.5ml eppendorf tubes.
For large constructs (BACs, PACs, cosmids, fosmids) please contact the facility first.
Sequencing turn-around time
Not mentioned
International orders
No information
GLP DNA sequencing
Not mentioned
Other DNA sequencing related services offered
- Fragment Analysis
Contact Information
Mr Steve Turner
DNA Sequencing Core
Main Building
Park Place
Cardiff CF10 3TL
Wales UK
Phone: +44(0)29 2087 6655
Email: TurnerSG@cf.ac.uk
Website: http://probe.biosi.cf.ac.uk/seq/default.php
Comments
Prices are not available on the website. The DNA sequencing core facility is available for use by external clients. Detailed guidelines are available on the website for primer design, sample preparation and submission. Also a list of standard primers with sequences is available on the website.