General Information
The Sequencing Facility is located within the Bio-Protection Research Centre, Burns Building, Lincoln University, New Zealand.
DNA Sequencing Instruments Used
- Applied Biosystems 3130xl
DNA Sequencing Prices
Capillary separation
-
$10 per sample
-
$9 per sample after 200 samples
Full sequencing
-
$13 per sample
-
$11 per sample after 200 samples
DNA Template Requirements
Single-stranded
- 25-50 ng /µl
Double-stranded (Plasmid DNA)
- 50-300 ng/µl
Cosmid, BAC
- 0.5-1.0 µg/µl
Bacterial genomic DNA
- 2-3 µg/µl
PCR Product:
-
100-200 bp | 1-3 ng/µl
-
200-500 bp| 5-20 ng/µl
-
500-1000 bp| 10-20 ng/µl
-
1000-2000 bp| 20-40 ng/µl
-
>2000 bp | 50 ng /µl
Primers
- 5 pmoles
Other Information
The facility offers four sequencing primers free of cost.
Sequencing turn-around time
24 – 48 hours
International orders
No information
GLP DNA sequencing
Not mentioned
Other DNA sequencing related services offered
-
Fragment Analysis
-
Genotyping
Contact Information
Sequencing Facility, Bio-Protection Research Centre
Burns Building
Lincoln University
Ellesmere Junction Road/Spring Road
Lincoln
Canterbury
New Zealand
Phone: +1 +64 3 325 3696
Email: sequence@lincoln.ac.nz
Website: http://www.lincoln.ac.nz/Services-facilities-and-support/Research-and-testing-services/Sequencing-and-genotyping/
Comments
Results are permanently stored in their original format and are available with permission to access them. Unused template is frozen (-20∫C) until collected or authorized to destroy. The Sequencing Facility is open between 9:30am and 2:30pm and samples received after 1pm will be processed following day.