General Information
The London Regional Genomics Centre has been established through generous support from the Robarts Research Institute that provides NGS service. The DNA Sequencing Facility at Robarts provides Sagner and other sequencing services.
DNA Sequencing Instruments Used
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Applied Biosystems 3730
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Ion Torrent PGM
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Illumina MiSeq
DNA Sequencing Prices
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Standard Sequencing reaction | $6
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Sequencing reaction with dGTP added | $7
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Fragment analysis and SNapShot | $6
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Large DNA (Genomic, BAC’s, Cosmids, Fosmid) | $20
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Commercial Sequencing | $12
DNA Template Requirements
Single Stranded DNA
- 20-50ng /ul
Double Stranded DNA
- 50-150ng /ul
BACs, PACs, YACs
- 0.4-1.0 ug
Bacterial Genomic DNA
- 2-3ug
PCR products
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100-200bp | 1-3ng /ul
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200-500bp | 3-10ng /ul
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500-1000bp | 5-10ng /ul
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1000-2000bp | 10-30ng /ul
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>2000bp | 40-100ng/ul
Primers
- 3.2 pmole
Other information
The facility provides the standard primers free of charge.
Sequencing turn-around time
48 hours
International orders
No information
GLP DNA sequencing
Not mentioned
Other DNA sequencing related services offered
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Next Generation Sequencing
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Library Preparation
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SNP Analysis
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Fragment Analysis
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Primer Walking
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Quantitative PCR
Contact Information
Dr. Robert Hegele
DNA Sequencing Facility
Robarts Research Institute
Room 4212
1151 Richmond St. N.,
London, Ontario
N6A 5B7
Canada
Phone: +1 (519) 931-5777
Fax: +1 (519) 931-5789
Email: sequencing@robarts.ca
Website: http://www.robarts.ca/facility-description
Comments
The facility operates on fee-for-service /cost recovery basis and so is able to provide sequencing for relatively low prices. Discounts are available for large volume projects. All samples are processed on a first come first served basis.