General Information
Established in 1993 as a part of NIEHS Center, UC Berkeley DNA Sequencing Facility serves all UC affiliated laboratories, government agencies and businesses providing DNA sequencing, genotyping, PCR cleanup and Plasmid preparation services.
DNA Sequencing Instruments Used
- Applied Biosystems 3730xl
DNA Sequencing Prices
On Campus
- Single-Tube Sequencing | $ 4.00 / sample
- Full Plate Sequencing | $240.00 / plate
Off Campus
- Single-Tube Sequencing | $ 6.26 / sample
- Full Plate Sequencing | $375.60 / plate
DNA Template Requirements
Single tube (13µl)
- 0.25µg DNA/5kb single stranded plasmid + 2 pmol primer
- 0.5µg DNA/5kb double stranded plasmid + 3.2 pmol primer
- 100ng DNA/1000bp PCR product + 0.8 pmol primer
Full plate submission (6µl)
- 250ng DNA/5kb double stranded plasmid + 1.6 pmol primer
- 50ng/1000 bp PCR product + 0.4 pmol primer
Other information
For templates larger than 10KB, please provide 1µg of template with 10pmol of primer in total volume of 13ul. Please make sure your primers have a melting temperature (Tm) between 52∞C and 60∞C
Sequencing Turn-around Time
24 hours
International orders
No information
GLP DNA sequencing
Not mentioned
Other DNA sequencing related services offered
- Fragment Analysis
- Genotyping
Contact Information
University of California, Berkeley
DNA Sequencing Facility
310 Barker Hall
Berkeley, CA 94720-3202
USA
Phone: +1 510-642-6383
Email: dnaseq@berkeley.edu
Website: http://mcb.berkeley.edu/barker/dnaseq/
Comments
They are testing a new sequencing protocol for cosmid, BACs, PACs, and Fosmids. They will sequence your sample for free of charge. If you have a protocol, they are ready to try it. An excellent facility committed to quality results. Highly recommended.
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