General Information
University of Pompeu Fabra (UPF) Genomics Core Facility was created in 1999 to give service to the research community with the most updated technology for DNA study in a joined effort. Recently the facility has acquired next-generation sequencers in 2011.
DNA Sequencing Instruments Used
- Applied Biosystems 3130xl
- Applied Biosystems 3730xl
- Illumina MiSeq
- Roche 454 GS Junior
DNA Sequencing Prices
Internal
1.70 euro per sample
External
4.00 euro per sample
DNA Template Requirements
Single-stranded
- 25-50 ng
Double-stranded
- 150-300 ng
Cosmid, BAC
- 0.5-1.0 µg
Bacterial genomic DNA
- 2-3 µg
PCR Products
- 100-200 bp | 1-3 ng
- 200-500 bp| 3-10 ng
- 500-1000 bp| 2-20 ng
- 1000-2000 bp| 10-40 ng
- >2000 bp | 40-100 ng
Primers
10uM
Other information
For a small volume samples (less than 50), prepare samples in tubes of 1.5ml eppendorf tubs
Sequencing turn-around time
Not mentioned
International orders
No
GLP DNA sequencing
Not mentioned
Other DNA sequencing related services offered
- Next Generation Sequencing
- Library Preparation
- Fragment Analysis
- Genotyping
- Real Time PCR
Contact Information
Genomic UPF Service
4th floor, office and laboratory 421 406
Biomedical Research Park (PRBB)
08003 Barcelona
Spain
Phone: +34 933160846
Email: genomica@upf.edu
Website: http://www.upf.edu/sct/en/genomica/presentacio/
Comments
The service is available only for University of Pompeu Fabra members. Generate read length in the range of 800-1000 bp. Once processed output files are transferred to a central server accessible from any university computer.
Return to the main DNA sequencing service reviews page