General Information
The Functional Genomics Center (FGC) is now the UR Genomics Research Center (URGRC). The URGRC is a core resource that supports all aspects of genomics research at the University of Rochester.
DNA Sequencing Instruments Used
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ABI 3730xl
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Illumina HiSeq2500
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Illumina MiSeq
DNA Sequencing Prices
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Standard Reaction – $5.50/ reaction ($450.00 per 96 well plate)
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Pre-Mixed Reaction – $5.50/ reaction ($450.00 per 96 well plate)
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Sequencing only – $3.50/ sample ($250.00 per 96 well plate)
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Cleanup and sequencing – $4.50/ sample ($350.00 per 96 well plate)
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Difficult Template (GC-rich, Hairpin, etc.) – $6.50/ sample
DNA Template Requirements
Plasmid DNA
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<10kb 400ng | 40ng/µl
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>10kb 2000ng | 100ng/µl
PCR Product
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100-200bp | 20ng | 2ng/µl
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200-500bp | 40ng | 4ng/µl
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500-1000bp | 80ng |8ng/µl
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1000-2000bp | 100ng |10ng/µl
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>2kb | 400ng |40ng/µl
Primers
- 20pmol |12µl
Some universal primers are available at no charge.
Other information
If you supply custom primers for sequencing, please submit them using the “Pre-mixed” option. They recommend eluting or re-suspending DNA in sterile Dnase/Rnase-free water, NOT Tris-EDTA (TE).
Sequencing Turn Around Times
24-48 hours
International orders
No information
GLP DNA sequencing
Not mentioned
Other DNA sequencing related services offered
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Next Generation Sequencing
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Library Preparation
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Quantitative PCR
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Genotyping
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Micro Array Analysis
Contact Information
Genomics Research Center
University of Rochester
James P. Wilmot Cancer Center
601 Elmwood Ave.
Rochester, NY 14642
USA
Phone: +1 585 275 7279
Fax: +1 585 427 9334
Email: urgenomics@urmc.rochester.edu
Website: http://www.urmc.rochester.edu/fgc/
Comments
All work performed by the University of Rochester Genomics Research Center (URGRC) should be acknowledged in scholarly publications, posters, and oral presentations.