General Information
The W.M. Keck Foundation Biotechnology Resource Laboratory was founded in 1980 as the Yale University School of Medicine Protein and Nucleic Acid Chemistry Facility. The Keck Biotechnology Resource Laboratory provides 113 genomic, proteomic, biostatistical, bioinformatics, and high performance computing technologies to hundreds of Yale and non-Yale investigators.
DNA Sequencing Instruments Used
- ABI 3730xl
- Ion Torrent PGM
DNA Sequencing Prices
- Premixed sample | $2.90 | $3.48 (External)
- Plate Sequencing | $1.50/well | $1.80/well (External)
DNA Template Requirements
Double stranded plasmids
- 500-600 ng
Cosmids
- 2000-4000 ng
PCR products
- 10-20 ng of PCR product for every 200 bp of product
Primers
- 2µl of 4 µM
Other information
The minimum number of samples for a single high volume plate is 48. All high volume plate submissions must be in red 96-well Eppendorf twin tec plates or Applied Biosystems Optical 96-well reaction plates.
Sequencing Turn Around Times
8 – 24 hours
International orders
No information
GLP DNA sequencing
Not Mentioned
Other DNA sequencing related services offered
- Individual tube sequencing
- High volume plate sequencing
- PCR purification
- Fragment analysis
Contact Information
Keck DNA Sequencing Lab
300 George St
Room 2127
New Haven, CT 06511
USA
Phone: +1 203 737 2566
Fax: +1 203 785 5586
Email: dnasequencing@yale.edu
Website: http://medicine.yale.edu/keck/dna/index.aspx
Comments
The Keck DNA Sequencing Facility provides competitive and timely DNA sequencing in an efficient and cost effective manner.
Return to the main DNA sequencing services review page.