misterious sequencing
Posted by: Daniel Xi (IP Logged)
Date: January 15, 2009 08:36AM

I have the following DNA sequence which gives perfect forward result but the reverse is messy. PCR product is a sharp single band. Either gel purification or SAP-IT is used before sequencing reaction. Can someone here point out the key? Thanks.

TTATNNGAATATCTATATAATTGATACATATTTTAATTATAAAAACTTAAGTAGTATTAAATATTGATATTTCTTTTTGTACAAACCTTTGTTAGTAATTTTAGTTCATATTCAGTTATACATTGTTTTT
GGATGTTTATGCAATCTTATTTAAATATTTTAAAAATAATTGTAATATACTATTTTGAGCACATTTTTGTGTCTCATTTA
CTTTATTCATTTATCATTGTTATCGTCCTTAGGAAAACGTTATTTTCCTTATTTGGAAAATGGACATAATGAAAATTACG
GAAGAAAGTTTGTACAGGGTAACTCTGTAGAAGTTGCCTTCCTTCCTGGCTGTGGTCTTCCAAATGAGCAGACCACAGTT
ACATGTATGGAGAATGGCTGGTCTCCTCCTTGCAGATGCATCCGTGTCACTA



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