misterious sequencing
Posted by:
Daniel Xi (IP Logged)
Date: January 14, 2009 04:36PM
I have the following DNA sequence which gives perfect forward result but the reverse is messy. PCR product is a sharp single band. Either gel purification or SAP-IT is used before sequencing reaction. Can someone here point out the key? Thanks.
TTATNNGAATATCTATATAATTGATACATATTTTAATTATAAAAACTTAAGTAGTATTAAATATTGATATTTCTTTTTGTACAAACCTTTGTTAGTAATTTTAGTTCATATTCAGTTATACATTGTTTTT
GGATGTTTATGCAATCTTATTTAAATATTTTAAAAATAATTGTAATATACTATTTTGAGCACATTTTTGTGTCTCATTTA
CTTTATTCATTTATCATTGTTATCGTCCTTAGGAAAACGTTATTTTCCTTATTTGGAAAATGGACATAATGAAAATTACG
GAAGAAAGTTTGTACAGGGTAACTCTGTAGAAGTTGCCTTCCTTCCTGGCTGTGGTCTTCCAAATGAGCAGACCACAGTT
ACATGTATGGAGAATGGCTGGTCTCCTCCTTGCAGATGCATCCGTGTCACTA