Re: Mixed signal in the middle of sequencing trace

I have encountered a situation in which a sequencing trace is of good quality and has single peaks but halfway through (about 200bp), the peaks suddenly become mixed ("peak under peak") all the way to the end. This was observed in a few other samples of the same sequencing batch. What could have been the problem? Thank you in advance.

This sound like an indel. Are you sequencing PCR products from a heterozygous individual?

Daniel Tillett
Nucleics Support

nucleics Wrote:
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> This sound like an indel. Are you sequencing PCR
> products from a heterozygous individual?

Yes. We finally understood why we got these results are you're right indeed. It was a heterozygous individual. Thanks!