PreviousNext
Forum ListMessage ListNew TopicSearchLog In
PCR Numerical.. Pls HELP
Posted by: Nimit (IP Logged)
Date: May 11, 2009 09:27AM

5 picomole of primer and 5 copy of a gene = given

you have 100% PCR efficiency. What is the maximum molecule
that can accumulate in 30 cycles of PCR..?

Options: Reply To This MessageQuote This Message
Re: PCR Numerical.. Pls HELP
Posted by: nucleics (IP Logged)
Date: September 08, 2009 09:37AM

This is a difficult question to answer as the amplification efficiency changes throught the whole PCR reaction. Assuming 100% efficiency the answer is 5 x 2^30. In practice, 5 x 1.8^30 is more reasonable.

Daniel Tillett
Nucleics Support

Options: Reply To This MessageQuote This Message


 Forum ListMessage ListSearchLog In
Your Name: 
Your Email: 
Subject: 
This is a moderated forum. Your message will remain hidden until it has been approved by a moderator or administrator

dna sequencing home | news about sequencing dna | about dna sequencing services | longtrace dna sequence service | peaktrace dna sequence basecaller | dna sequencing software, service, reagents | dna sequencing services | dna sequencing support | contact us about sequencing dna | resources for dna sequencing service, reagent, software | dna sequence news
©Nucleics 2007