Re: PCR product storage

Once I have a purified PCR product, what temperature is best for storage prior to sequencing? How long can it be stored at that temp?

It depends what the PCR products are stored in. If it is in water it is best to freeze the sample unless you are going to use them within a few hours.

I prefer to use 10/0.1 TE [10mM Tris, 0.1 mM EDTA, pH 8.5] to store my PCR products. The tris helps stop the solution from becoming acidic (cause by CO2 being absorbed from the air) and the small amount of EDTA doesn't interfere with the sequencing reaction. If you use this then you can just store at 4.deg.C. I have found that stored in 10/0.1 TE the PCR products will last for years in the fridge.

Daniel Tillett
Nucleics Support