What DNA sequencing service facility should I use?
Posted by: nucleics (IP Logged)
Date: October 03, 2006 06:30PM

This is a question we often get asked (it is one of the reasons we started doing the reviews). It is basically an impossible question to answer as it depends on you needs and location. The best way to approach the question is deciding what are your essential requirements, eliminating all the facilities that don't fit, then selecting on price, and then on distance from you.

If you would like advice post what your essential requirements are in this forum and we will try to provide you with a list of five facilities that meet these requirements.

Daniel Tillett
Nucleics Support

Re: What DNA sequencing service facility should I use?
Posted by: rgrant (IP Logged)
Date: October 04, 2006 06:34PM

What is the cheapest place to get sequencing done in the USA?

Re: What DNA sequencing service facility should I use?
Posted by: nucleics (IP Logged)
Date: October 09, 2006 11:47PM

This really depends on what services you want and how many samples that you want to process at a time. Some sequencing facilities are very cheap if you send in a minimum of 96 samples in a tray, while others are much better for only a few samples.

The other major area that can make quite a difference in price is if you want to do your own reactions or not. can you provide more information of what you want?

Daniel Tillett
Nucleics Support

Re: What DNA sequencing service facility should I use?
Posted by: rgrant (IP Logged)
Date: October 11, 2006 08:53PM

Thanks for your help Daniel. I want to do my own reactions and I have around 300 samples that I want to sequence. Is that enough information?

Re: What DNA sequencing service facility should I use?
Posted by: nucleics (IP Logged)
Date: October 17, 2006 10:10PM

Not a problem rgrant :)

There are quite a few that are quite low cost facilities if you want to send ready-to-run samples. Probably the cheapest is UCLA Core Facility at $150 for a 96 well tray which is only a $1.56 a trace. Have a look through the reviews we have done (www.nucleics.com/DNA_sequencing_support/sequencing-service-reviews.html) if you haven't already.

Cheers

Daniel Tillett
Nucleics Support

Re: What DNA sequencing service facility should I use?
Posted by: Paola Navarrete (IP Logged)
Date: June 24, 2008 12:11PM

Hello,
I have 20 PCR products (about 600 pb) to be purified and sequenced. I live in Chile, southamerica, and I want to know What is the cheapest and rapid place to get sequencing?
thanks

Paola

Re: What DNA sequencing service facility should I use?
Posted by: nucleics (IP Logged)
Date: June 24, 2008 05:49PM

Hi Paola

I don't know of any sequencing facilities in South America, so I would suggest using any of the facilities in the USA that are shown in red on the main sequencing facility review pages. These are customers of ours that are using LongTrace or PeakTrace and that I know generate good quality data - speed is not much help if what you get back is not good quality :)

Daniel Tillett
Nucleics Support



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