<?xml version="1.0" encoding="iso-8859-1" ?>
<rss version="2.0">
  <channel>
    <title>General Questions</title>
    <link>http://www.nucleics.com/forum/list/11.html</link>
    <description><![CDATA[For any general questions about Nucleics or our products]]></description>
    <language>EN</language>
    <pubDate>Mon, 14 Dec 2009 11:33:06 -0500</pubDate>
    <lastBuildDate>Mon, 14 Dec 2009 11:33:06 -0500</lastBuildDate>
    <category>General Questions</category>
    <generator>Phorum 5.1.22</generator>
    <ttl>600</ttl>
    <item>
      <title>Re: ABI 310 - Discussion/ troubleshooting/ community....</title>
      <link>http://www.nucleics.com/forum/read/11/2791/2817.html#msg-2817</link>
      <author>j</author>
      <description><![CDATA[I hear the same thing...hopefully not a big deal?  I got some arcing when I had extra buffer around the septas holder in position one.  Blue light and sparks were coming out and every time I hear that clicking sound I think it's happening again...but I look and I don't see anything.]]></description>
      <category>General Questions</category>
      <guid isPermaLink="true">http://www.nucleics.com/forum/read/11/2791/2817.html#msg-2817</guid>
      <pubDate>Mon, 14 Dec 2009 11:33:06 -0500</pubDate>
    </item>
    <item>
      <title>ABI 310 - Discussion/ troubleshooting/ community....</title>
      <link>http://www.nucleics.com/forum/read/11/2791/2791.html#msg-2791</link>
      <author>Mike</author>
      <description><![CDATA[Not sure if this is an appropriate forum but here goes anyway....

I'm using an old ABI 310 sequencer - which it seems is so dated that there are no discussion groups/forums around.  Now I'm sure I'm not the only one still using one of these-  any want to point me in the direction of a forum?  Or can we start one? Is anyone there?

I have hit a few problems with the intstrument which I've overcome, a few which I haven't -  lets try and find some more users.

Right now everything seems to be working fine however I've noticed a new clicking type sound when the electrophoresis is running-  sounds almost like electrical shorting/arching - not that I can find any apparent cause....  Any thoughts?]]></description>
      <category>General Questions</category>
      <guid isPermaLink="true">http://www.nucleics.com/forum/read/11/2791/2791.html#msg-2791</guid>
      <pubDate>Thu, 12 Nov 2009 12:10:03 -0500</pubDate>
    </item>
    <item>
      <title>Re: Welcome to The Nucleics Forums</title>
      <link>http://www.nucleics.com/forum/read/11/34/2414.html#msg-2414</link>
      <author>Milena Soares</author>
      <description><![CDATA[Hi,

I would like to know how can I send PCR products to your sequences facilities (Brazil--&gt; Korea). How can the samples must be directed: Congealed, cooled or the ambient temperature? and I would like to know which the prices for sequence.

Kind regards,

Milena Soares
Patology and Molecular Biology Laboratory
Oswald Cruz Foundation-FIOCRUZ
Salvador-Bahia-Brazil
phone: +55 71 3176-2265/2311/2301
fax: +55 71 3176-2289]]></description>
      <category>General Questions</category>
      <guid isPermaLink="true">http://www.nucleics.com/forum/read/11/34/2414.html#msg-2414</guid>
      <pubDate>Sat, 28 Mar 2009 22:30:24 -0400</pubDate>
    </item>
    <item>
      <title>Re: DNA Analysis software</title>
      <link>http://www.nucleics.com/forum/read/11/789/794.html#msg-794</link>
      <author>nucleics</author>
      <description><![CDATA[Hi Emily

I think you are right - I have not seen any other software around like it either :)]]></description>
      <category>General Questions</category>
      <guid isPermaLink="true">http://www.nucleics.com/forum/read/11/789/794.html#msg-794</guid>
      <pubDate>Tue, 20 May 2008 22:37:13 -0400</pubDate>
    </item>
    <item>
      <title>Re: DNA Analysis software</title>
      <link>http://www.nucleics.com/forum/read/11/789/793.html#msg-793</link>
      <author>EmilyT</author>
      <description><![CDATA[Hey Daniel,

Thanks for that, I managed to track down the exact same company and software shortly after posting. Doesn't appear to be any other packages with the same feature.

Cheers,
Emily]]></description>
      <category>General Questions</category>
      <guid isPermaLink="true">http://www.nucleics.com/forum/read/11/789/793.html#msg-793</guid>
      <pubDate>Tue, 20 May 2008 21:47:37 -0400</pubDate>
    </item>
    <item>
      <title>Re: DNA Analysis software</title>
      <link>http://www.nucleics.com/forum/read/11/789/790.html#msg-790</link>
      <author>nucleics</author>
      <description><![CDATA[Hi Emily

Yes I have seen this software before too - the same as you I can't remember what it is called. I will take a look around and if I can find it I will post it here.

Edit. Found it. The software is called Mutation Surveyor and is sold by softgenetics.]]></description>
      <category>General Questions</category>
      <guid isPermaLink="true">http://www.nucleics.com/forum/read/11/789/790.html#msg-790</guid>
      <pubDate>Tue, 20 May 2008 01:07:49 -0400</pubDate>
    </item>
    <item>
      <title>DNA Analysis software</title>
      <link>http://www.nucleics.com/forum/read/11/789/789.html#msg-789</link>
      <author>EmilyT</author>
      <description><![CDATA[Does any know of Sequence analysis software that can deconvulute heterozygous diploid sequence downstream of small INDELs. We have a vague recollection of seeing a package which claimed to be able to do this, but have had no luck tracking down anything like this.]]></description>
      <category>General Questions</category>
      <guid isPermaLink="true">http://www.nucleics.com/forum/read/11/789/789.html#msg-789</guid>
      <pubDate>Mon, 19 May 2008 23:47:16 -0400</pubDate>
    </item>
    <item>
      <title>ABI 3130 &amp; CEQ 8800 comparison</title>
      <link>http://www.nucleics.com/forum/read/11/783/783.html#msg-783</link>
      <author>Cesar Bedoya</author>
      <description><![CDATA[We are buying our first DNA sequencer, we plan to use for analysis PCR fragments, Viral genomes, VIH genotyping &amp; microsatellites. Please somebody could me advise which is the best machine for us, ABI 3130 or CEQ 8800? 

Regards

Cesar]]></description>
      <category>General Questions</category>
      <guid isPermaLink="true">http://www.nucleics.com/forum/read/11/783/783.html#msg-783</guid>
      <pubDate>Fri, 09 May 2008 14:54:32 -0400</pubDate>
    </item>
    <item>
      <title>sequencing trouble for the third time!</title>
      <link>http://www.nucleics.com/forum/read/11/408/408.html#msg-408</link>
      <author>anandi</author>
      <description><![CDATA[I could really do with some help! I have general primers that ought to bind to the cytochrome b mitochondrial gene in bos taurus (cattle). now, the forward sequences have worked alright 2 out of 3 times, the reverse hasn't workerd at all except in one single sample during the very first run!? i know my initial PCR is ok so it has to be the sequencing cleanup or the sequencing pcr reaction. i get either no run at all, just 4 or so unidentified nucleotides or i get huge dye blobs and G due peaks that stretch for many bases. could it be the cleanup step when my forward reactions work well? could it be the primer? some outside thoughts would be appreciated! feel free to E-mail me at s24061621@tuks.co.za

kind regards
anandi]]></description>
      <category>General Questions</category>
      <guid isPermaLink="true">http://www.nucleics.com/forum/read/11/408/408.html#msg-408</guid>
      <pubDate>Thu, 28 Feb 2008 07:39:01 -0500</pubDate>
    </item>
    <item>
      <title>How to post a question</title>
      <link>http://www.nucleics.com/forum/read/11/39/39.html#msg-39</link>
      <author>nucleics</author>
      <description><![CDATA[You do not need to register if you want to post a question or reply, however, all posts from non-registered users need to be moderated before they appear. Since we are located in Australia (GMT +10 hours) this can mean that it may take a little time for your message to appear if you happen to post while we are sleeping.

To register click on the forum login menu button (this should be at the top of the forum next to the search menu button). Once there cick on the link which say &quot;Not registered&quot; (should be below the login boxes). You will need to supply a working email address as the forum activation link will be mailed to this email address. Before you can post you will need to click on the link in this email. This will activate your account.

We will not send you any emails to this address except those that relate to this forum.

Update

I have recently being looking through the user accounts and there are quite a number of people who tried to register accounts but never activated them. You MUST activate your account before you can post so remember to use a email account that works.]]></description>
      <category>General Questions</category>
      <guid isPermaLink="true">http://www.nucleics.com/forum/read/11/39/39.html#msg-39</guid>
      <pubDate>Tue, 26 Sep 2006 19:10:32 -0400</pubDate>
    </item>
    <item>
      <title>Welcome to The Nucleics Forums</title>
      <link>http://www.nucleics.com/forum/read/11/34/34.html#msg-34</link>
      <author>nucleics</author>
      <description><![CDATA[Welcome to the Nucleics forums. 

This is the place to ask question about us or any of our DNA sequencing products. We have also provided sections where you can ask more general molecular biology protocol questions, or questions about how you can improve your DNA sequencing reactions.]]></description>
      <category>General Questions</category>
      <guid isPermaLink="true">http://www.nucleics.com/forum/read/11/34/34.html#msg-34</guid>
      <pubDate>Mon, 25 Sep 2006 22:24:31 -0400</pubDate>
    </item>
  </channel>
</rss>
