<?xml version="1.0" encoding="iso-8859-1" ?>
<rss version="2.0">
  <channel>
    <title>dLUTE SEQ Forum</title>
    <link>http://www.nucleics.com/forum/list/7.html</link>
    <description><![CDATA[Discussion about the dLUTE SEQ and dLUTE SEQ CF DNA sequencing BigDye dilution reagents]]></description>
    <language>EN</language>
    <pubDate>Wed, 08 Apr 2009 05:36:10 -0400</pubDate>
    <lastBuildDate>Wed, 08 Apr 2009 05:36:10 -0400</lastBuildDate>
    <category>dLUTE SEQ Forum</category>
    <generator>Phorum 5.1.22</generator>
    <ttl>600</ttl>
    <item>
      <title>Re: Does dlute work with amersham machines?</title>
      <link>http://www.nucleics.com/forum/read/7/61/2432.html#msg-2432</link>
      <author>nucleics</author>
      <description><![CDATA[Hi Zoltan

No just use your normal injection. Once the sample is cleaned up it is the same as a normal sample.]]></description>
      <category>dLUTE SEQ Forum</category>
      <guid isPermaLink="true">http://www.nucleics.com/forum/read/7/61/2432.html#msg-2432</guid>
      <pubDate>Wed, 08 Apr 2009 05:36:10 -0400</pubDate>
    </item>
    <item>
      <title>Re: Does dlute work with amersham machines?</title>
      <link>http://www.nucleics.com/forum/read/7/61/2431.html#msg-2431</link>
      <author>Zoltán Gyuris</author>
      <description><![CDATA[Hi, we have two Amersham machines. If I use dLUTE SEQ, should I modify the parameters of electrokinetic injection?

Thanks

Zoltán]]></description>
      <category>dLUTE SEQ Forum</category>
      <guid isPermaLink="true">http://www.nucleics.com/forum/read/7/61/2431.html#msg-2431</guid>
      <pubDate>Wed, 08 Apr 2009 05:13:33 -0400</pubDate>
    </item>
    <item>
      <title>Re: What is included in dLUTE SEQ CF Kit?</title>
      <link>http://www.nucleics.com/forum/read/7/1826/1827.html#msg-1827</link>
      <author>nucleics</author>
      <description><![CDATA[No neither of these items are included in the dLUTE SEQ kits. The reason for this is both contain ethanol and shipping any ethanol containing solution via air is fraught with legal difficulties. We do provide the formula to make up these solutions - they just contain basic molecular biology chemicals which all labs should have.]]></description>
      <category>dLUTE SEQ Forum</category>
      <guid isPermaLink="true">http://www.nucleics.com/forum/read/7/1826/1827.html#msg-1827</guid>
      <pubDate>Thu, 30 Oct 2008 09:12:36 -0400</pubDate>
    </item>
    <item>
      <title>What is included in dLUTE SEQ CF Kit?</title>
      <link>http://www.nucleics.com/forum/read/7/1826/1826.html#msg-1826</link>
      <author>MC</author>
      <description><![CDATA[Does dLUTE SEQ Precipitation Solution and dLUTE SEQ Wash Solution are included in kit?]]></description>
      <category>dLUTE SEQ Forum</category>
      <guid isPermaLink="true">http://www.nucleics.com/forum/read/7/1826/1826.html#msg-1826</guid>
      <pubDate>Thu, 30 Oct 2008 07:21:46 -0400</pubDate>
    </item>
    <item>
      <title>Re: Can I use dLUTE with ABI 3130?</title>
      <link>http://www.nucleics.com/forum/read/7/69/317.html#msg-317</link>
      <author>nucleics</author>
      <description><![CDATA[Yes you can. You don't need a robot to set up dLUTE SEQ as you can also do it by hand if you want.]]></description>
      <category>dLUTE SEQ Forum</category>
      <guid isPermaLink="true">http://www.nucleics.com/forum/read/7/69/317.html#msg-317</guid>
      <pubDate>Sun, 24 Jun 2007 21:15:41 -0400</pubDate>
    </item>
    <item>
      <title>Re: Modification to dLUTE SEQ thermocycling conditions</title>
      <link>http://www.nucleics.com/forum/read/7/203/204.html#msg-204</link>
      <author>JohnH</author>
      <description><![CDATA[I will give this a try.]]></description>
      <category>dLUTE SEQ Forum</category>
      <guid isPermaLink="true">http://www.nucleics.com/forum/read/7/203/204.html#msg-204</guid>
      <pubDate>Mon, 07 May 2007 22:33:00 -0400</pubDate>
    </item>
    <item>
      <title>Modification to dLUTE SEQ thermocycling conditions</title>
      <link>http://www.nucleics.com/forum/read/7/203/203.html#msg-203</link>
      <author>nucleics</author>
      <description><![CDATA[In the process of developing dLUTE SEQ CF we have discovered a simple change to the thermocycling conditions greatly improves the robustness of the dLUTE reaction. 

This change is the addition of a 3 minute 75˚C preincubation step before the main cycling steps. This step allows the dLUTE phases to separate before the DNA polymerase in the sequencing mixture is exposed to denaturing temperatures. This appears to prevent the polymerase from inactivating when exposed to the large surface area of the inert phase in its intact form.]]></description>
      <category>dLUTE SEQ Forum</category>
      <guid isPermaLink="true">http://www.nucleics.com/forum/read/7/203/203.html#msg-203</guid>
      <pubDate>Mon, 07 May 2007 02:07:32 -0400</pubDate>
    </item>
    <item>
      <title>Re: dLUTE CF announcement</title>
      <link>http://www.nucleics.com/forum/read/7/109/202.html#msg-202</link>
      <author>nucleics</author>
      <description><![CDATA[We have finished developing the dLUTE SEQ CF formulation. We have been able to keep the dLUTE component the same as the conventional dLUTE SEQ reagent and achieve the change by just modifying the phase indicator solution. Consequently, dLUTE SEQ CF will be priced the same as the standard dLUTE SEQ reagent. 

We should have a description and ordering details up on our web site in the next few days, however, if you wish to order it now just order the standard dLUTE SEQ reagent and make a note that you wish to receive the CF version.]]></description>
      <category>dLUTE SEQ Forum</category>
      <guid isPermaLink="true">http://www.nucleics.com/forum/read/7/109/202.html#msg-202</guid>
      <pubDate>Mon, 07 May 2007 02:00:50 -0400</pubDate>
    </item>
    <item>
      <title>dLUTE CF announcement</title>
      <link>http://www.nucleics.com/forum/read/7/109/109.html#msg-109</link>
      <author>nucleics</author>
      <description><![CDATA[We have developed an new version of the dLUTE SEQ product designed for small scale users and core facilities processing user supplied DNA (dLUTE CF). This version, which is in beta testing, offers a simpler protocol and more importantly improved robustness with DNA of variable quality and concentration. If you are interested in being a beta tester of dLUTE CF then please email our support team.

Daniel]]></description>
      <category>dLUTE SEQ Forum</category>
      <guid isPermaLink="true">http://www.nucleics.com/forum/read/7/109/109.html#msg-109</guid>
      <pubDate>Sun, 18 Mar 2007 19:01:49 -0400</pubDate>
    </item>
    <item>
      <title>Re: How do I order dlute?</title>
      <link>http://www.nucleics.com/forum/read/7/73/74.html#msg-74</link>
      <author>nucleics</author>
      <description><![CDATA[There are two ways of ordering. The simplest is to use our secure online ordering system. There is a link to this from the dLUTE SEQ information page. You will need an credit card to use this system. The alternative is to email our support staff with your order. You will need to use this approach if you want to use a purchase order.]]></description>
      <category>dLUTE SEQ Forum</category>
      <guid isPermaLink="true">http://www.nucleics.com/forum/read/7/73/74.html#msg-74</guid>
      <pubDate>Wed, 11 Oct 2006 21:51:07 -0400</pubDate>
    </item>
    <item>
      <title>How do I order dlute?</title>
      <link>http://www.nucleics.com/forum/read/7/73/73.html#msg-73</link>
      <author>maslunka</author>
      <description><![CDATA[Can you tell me how I can order the dlute seq reagent?]]></description>
      <category>dLUTE SEQ Forum</category>
      <guid isPermaLink="true">http://www.nucleics.com/forum/read/7/73/73.html#msg-73</guid>
      <pubDate>Tue, 10 Oct 2006 02:07:03 -0400</pubDate>
    </item>
    <item>
      <title>Can I use dLUTE with ABI 3130?</title>
      <link>http://www.nucleics.com/forum/read/7/69/69.html#msg-69</link>
      <author>rgrant</author>
      <description><![CDATA[Can I use dlute seq with a 3130? I don't have a robot to set up the reactions.]]></description>
      <category>dLUTE SEQ Forum</category>
      <guid isPermaLink="true">http://www.nucleics.com/forum/read/7/69/69.html#msg-69</guid>
      <pubDate>Tue, 10 Oct 2006 00:30:39 -0400</pubDate>
    </item>
    <item>
      <title>Re: Does dlute work with amersham machines?</title>
      <link>http://www.nucleics.com/forum/read/7/61/62.html#msg-62</link>
      <author>nucleics</author>
      <description><![CDATA[Yes it works fine. We have not tested it as much as with BigDye, but we did test it in the past to make sure that it would work fine.]]></description>
      <category>dLUTE SEQ Forum</category>
      <guid isPermaLink="true">http://www.nucleics.com/forum/read/7/61/62.html#msg-62</guid>
      <pubDate>Wed, 04 Oct 2006 03:26:51 -0400</pubDate>
    </item>
    <item>
      <title>Does dlute work with amersham machines?</title>
      <link>http://www.nucleics.com/forum/read/7/61/61.html#msg-61</link>
      <author>Muz</author>
      <description><![CDATA[Does the dlute seq work with the megabase machines&gt; What about the amersham sequencing chemistry?]]></description>
      <category>dLUTE SEQ Forum</category>
      <guid isPermaLink="true">http://www.nucleics.com/forum/read/7/61/61.html#msg-61</guid>
      <pubDate>Tue, 03 Oct 2006 22:35:10 -0400</pubDate>
    </item>
    <item>
      <title>Welcome to the dLUTE SEQ forum</title>
      <link>http://www.nucleics.com/forum/read/7/35/35.html#msg-35</link>
      <author>nucleics</author>
      <description><![CDATA[Welcome to the dLUTE SEQ forum. This is the place to ask questions about or seek advice on how to best use the dLUTE SEQ sequencing chemistry dilution reagent.]]></description>
      <category>dLUTE SEQ Forum</category>
      <guid isPermaLink="true">http://www.nucleics.com/forum/read/7/35/35.html#msg-35</guid>
      <pubDate>Mon, 25 Sep 2006 22:26:22 -0400</pubDate>
    </item>
  </channel>
</rss>
