General Information
The DNA Sequencing Facility based in the School of Life Sciences within University of Nottingham has provided comprehensive DNA sequence analysis service for over 20 years.
DNA Sequencing Instruments Used
- Applied Biosystems 3130xl
DNA Sequencing Prices
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Full Sequencing | £5.00 per reaction
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Run only | £2.95 per reaction
DNA Template Requirements
Plasmids
70-150 ng/µl
PCR Product
-
100-200 bp | 1-3 ng/µl
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200-500 bp| 3-10 ng/µl
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500-1000 bp| 2-20 ng/µl
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1000-2000 bp| 10-40 ng/µl
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more than 2000 bp | 40-100 ng /µl
Primers
- 10pmole/µl
Other information
Universal primers which are commonly found in cloning vectors are provided free of charge.
Sequencing turn-around time
24 hours
International orders
No information
GLP DNA sequencing
Not mentioned
Other DNA sequencing related services offered
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Next Generation Sequencing
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Fragment Analysis
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Genotyping
Contact Information
Matthew Carlile
DNA Sequencing Laboratory
School of Life Sciences
D95, Medical School
Queens Medical Centre
Nottingham
NG7 2UH
UK
Phone: +44 0115 8231480 | +44 0115 82 31480
Email: Matthew.Carlile@nottingham.ac.uk
Website: http://www.nottingham.ac.uk/life-sciences/facilities/dna-sequencing/index.aspx
Comments
Sequences are run over night to facilitate next day delivery. Deep Seq is the Next Generation Sequencing Facility.