General Information
The Duke University Marine Laboratory is place where scientists work at forefront of understanding marine environmental systems, their conservation, and governance. The MCMF is a shared-use facility at Duke Marine Lab which supports almost state-of-the-art equipment.
DNA Sequencing Instruments Used
- ABI 3730
DNA Sequencing Prices
A. ABI DNA Analyzer Plate Charge: $100 per plate
B. Reagents/Consumables: | Reagents | Reagents/Cons. | |
1) PCR Cleanup/Big Dye Rxns/Seq Cleanup | $120 | $160 | |
2) PCR to Sequencing | $140 | $190 | |
3) Microsatellites (PCR, marker) | $135 |
Includes plate charge:
- Consumables include tips and 96-well plates
- PCR Cleanup: enzymatic cleanup using Exonuclease I (NEB) and NTPhos (Epicentre)
- Seq Cleanup: AMPure Magnetic Beads (Agencourt) following MCMF standard protocols
C. Sequencing Service: $6 per sequencing reaction
DNA Template Requirements
- Microsatellites: must provide your own primers (labelled)
- All reactions must be performed using standard MCMF protocols
- Standard universal primers (M13 Forward and Reverse, T3, T7, LCOI, HCOI) will be provided by the MCMF
- Customer must provide DNA template (PCR product or purified plasmid) and primers (if non-universal primers are required)
- Sequencing will be performed on a weekly basis, samples submitted by Tuesday should be completed by the following Friday
- Contact staffs for more information
Sequencing Service List
- PCR Clean-up
- PCR to Sequencing
- Microsatellite
Sequencing turn-around time
Not mentioned
International orders
No
Contact Information
Address:
Duke University Marin Laboratory – Division of Marine Science and Conservation
135 Duke Marine Lab Road, Beaufort, NC 28516
MCMF Director – Dr. Tom Schultz – Email: tom.schultz@duke.edu – Tel: 252-504-7641
Website: https://nicholas.duke.edu/marinelab/about/facilities/marine-conservation-molecular-facility-mcmf
Comments
There is not much information in the website. Users have to contact to Dr. Tom Schultz to discuss about theirs samples. This facility might not be available if customers have a large number of samples.
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