General Information
ACTGene is a Brazilian company stablished in 2008 that providing DNA Sanger sequencing service.
DNA Sequencing Instruments Used
- Applied Biosystems 3500
DNA Sequencing Prices
Small Scale
- Samples supplied without BigDye | R$22 per sample
- Samples supplied with BigDye | R$15 per sample
- R$28 per sample needs to be run with primer service
Large scale
- 1 ~ 191 samples | R$22 per sample
- 192 ~ 383 samples | R$21.45 per sample
- 384 ~ 767 samples | R$20.9 per sample
- 768 samples ~ | R$19.80 per sample
DNA Template Requirements
Plasmids
- Tube | 100 – 250 ng/ µl | 6 µl minimum
- Plate | 50 – 125 ng/ µl | 2.5 µl minimum
PCR products
- Tube | 30 – 60 ng/ µl | 6µl minimum
- Plate | 15 – 30 ng/ µl | 2.5 µl minimum
Primers
- Final primer concentration : 4.5 pmol
- Universal primers are available from the facility.
Other information
Sequencing turn-around time
- Up to 15 days
International orders
- No information
GLP DNA sequencing
- No information
Other DNA sequencing related services offered
- No information
Contact Information
ACTGene Analyser Molecular Ltd
Rua Gustavo Valente, 69
Bairro Bela Vista
CEP 94810-250-Alvorada, RS
Brazil
https://ludwigbiotec.com.br/loja/
Contact person:
Jordana Ludwig or Thais Lopes
email: actgene@ludwigbiotec.com.br
Comments
They claim a read length of Sanger sequencing can be obtained from 700-1200bp.
They supply their services with a competitive price and if you are regular customers, you might have a good discount. Besides, 2 samples are run for free if you are new customer. The turn around times are not fast, but given they have a 3500 this is not surprising.
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