General Information
The Centre of Genomics Sciences (formerly known as Genome Research Centre) was established to provide leadership in genome research in Hong Kong and the region by developing expertise and infrastructure for studies in genomics, proteomics and bioinformatics.
The Centre offers professional core services with advanced high-throughput technology platforms. Other fundamental tools such as DNA sequencer, real-time PCR, bioanalyzer … are also provided.
DNA Sequencing Instruments Used
- ABI3730XL
- HiSeq 1500
- MiSeq
- PacBio
DNA Sequencing Price
Full Service Sequencing (cycle sequence reaction, purification, capillary electrophoresis)
HKU Academic Research:
- Template-Primer Premixed | HK$31 per sample
- Full plate (90 samples) | HK$2,350
Local Academic Research
- Template-Primer Premixed | HK$38 per sample
- Full plate (90 samples) | HK$2,800
Purification and Run (purification, capillary electrophoresis)
- HKU academic research | HK$28 per sample
- Local academic research | HK$34 per sample
DNA template requirement
- Full service sequencing | 20µl
- Purification and Run | 15µl
- Coloured samples are unacceptable
- less than 8 samples | samples have to be submitted in 0.2ml microtubes
- 8~32 samples | samples have to be submitted in 8-well strip tubes
- more than 32 samples | samples have to be submitted in 96-well plate
dsDNA, Plasmids
150-300ng
PCR products
- 100~200bp | 1~3ng
- 200~500bp | 3~10ng
- 500~1000bp | 5~20ng
- 1000~2000bp | 10~40ng
- >2000bp | 20~50ng
ssDNA
25~50ng
Cosmid, BAC
500~1000ng
Bacterial genomic DNA
2000~3000ng
Primers
- 1µl of 5uM sequencing primer
- Common universal primers are provided free. Primer orders are available
Sequencing turn-around time
48 hours from arrival time of samples
International orders
No, only HKU academic research or local academic research
Other DNA sequencing related services offered
- Next Generation Sequencing
- GeneScan Fragment Analysis
Contact Information
Address: Center of Genomics Sciences
6th floor, the Hong Kong Jockey Club Building for Interdisciplinary Research
5 Sassoon Road, Pokfulam, Hong Kong
Email: sangerseq.cgs@hku.hk
Website: http://cgs.hku.hk/portal/index.php/dna-sequencing
Comments
The service promises a high quality and accuracy of read length up to 1000bp. If you are local non-HKU academic users, please use the Yamato Transport delivery for on-site sample collection.
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