General Information
Wyzer Biosciences, Inc. was founded in 2011 and is located in Cambridge, MA, the home of Harvard, MIT and many other world-class educational and biotech institutions. Through Their expertise in molecular biology they provide services to partners and collaborators in industry and academia that will aid them in ongoing research.
DNA Sequencing Instruments Used
- ABI 3730xl
- Illumina MiSeq
- Thermo-Fisher IonTorrent
DNA Sequencing Prices
- Contact for a quote.
DNA Template Requirements
Plasmids
Insert Size*
(in kbp) |
ng of Plasmid DNA
(minimum/optimal) |
0.2 – 0.8 | 100/500 |
0.8 – 1.6 | 200/1000 |
1.6 – 2.4 | 300/1500 |
2.4 – 3.2 | 400/2000 |
3.2 – 4.0 | 500/2500 |
4.0 – 4.8 | 600/3000 |
4.8 – 5.6 | 700/3500 |
5.6 – 6.4 | 800/4000 |
6.4 – 7.2 | 900/4500 |
PCR products
Insert Size*
(in kbp) |
ng of PCR Fragment
(minimum/optimal) |
0.2 – 0.8 | 10/50 |
0.8 – 1.6 | 20/100 |
1.6 – 2.4 | 30/150 |
2.4 – 3.2 | 40/200 |
3.2 – 4.0 | 50/250 |
4.0 – 4.8 | 60/300 |
4.8 – 5.6 | 70/350 |
5.6 – 6.4 | 80/400 |
6.4 – 7.2 | 90/450 |
BAC’s
- Between 100-150 kbp submit 1000 ng of DNA per tube/well
Cosmids
- roughly 40 kbp submit 1000 ng of DNA per tube/well
Primers
- 1 μl of 5 μM ( = 5 pmol/μl) primer per well
Other information
- Wyzer bio has developed a number of proprietary protocols to deal with problematic DNA templates. Inform Wyzer bio during sample submission so they can perform analysis of samples appropriately.
Sequencing Turn Around Times
- Not listed
International orders
- Not Listed
GLP DNA sequencing
- Not Listed
Other DNA sequencing related services offered
- Next generation sequencing is offered through Wyzer Biosciences though their Illumina and Thermo Fisher platfroms.
Contact Information
Wyzer Biosciences
85 Bolton Street, Suite 120
Cambridge, MA 02140
617-945-9697 (phone)
617-945-9698 (fax)
www.wyzerbio.com
Comments
Wyzer offers a complete service from reaction to primer design and in depth analysis of your results. They are more than happy to answer any questions and cater to your Sequencing project needs.
Return to the main DNA sequencing services review page.