01 · What it is
A better basecaller for every ABI sequencer
PeakTrace is a Sanger DNA sequencing basecaller and trace processor. It gives better basecalls and improves the appearance and read length of DNA sequencing traces by up to 50%.
It works with all ABI sequencers, including the 377, 310, 3700, 3730, 3730xl, 3100, 3130 and 3500, and you only pay for the traces it improves.
How we validated it
02 · On that trace, aligned to the true sequence
1,540
bases aligned with PeakTrace, against 1,379 with KB
1,251
first basecall error with PeakTrace, against base 1,084 with KB
1,486
bases at a 1% error rate with PeakTrace, against 1,275 with KB
03 · Choose your setup
Four ways to run PeakTrace
As a rule, Auto PeakTrace RP costs less than PeakTrace:Box below about 150,000 traces a year. See prices
04 · Low risk
Pay for results, not for failed reactions
- You are only charged for traces that PeakTrace improves.
- A 60 day, full money back guarantee lets you trial it in production.
- PeakTrace can rescue some low quality traces, saving a re-run.
- Used another way, it can raise instrument throughput by up to 300% for short reads.
05 · Questions
Common questions about PeakTrace
More answers in the PeakTrace FAQ, or ask on the forum.
How is PeakTrace different from KB or phred?
PeakTrace is the next step in basecalling, in the same way KB improved on phred and the original ABI basecaller. It gives longer reads, more high quality bases and better looking traces than KB.
Can I trust the sequence data from PeakTrace?
Yes. We have validated PeakTrace so that its quality values match the true error rate. If you trust the KB basecaller, you can trust PeakTrace too.
Am I charged for traces PeakTrace does not improve?
No. Apart from a couple of unusual cases, you only pay for traces that PeakTrace improves, so failed reactions cost you nothing.
Can I try PeakTrace for free?
Yes. PeakTrace Online Pro gives sequencing facilities a week of the full software, up to 250 traces a day. Register when you have a good set of samples ready to process.
Will you keep my traces?
No. We do not examine paying customers’ data without their permission. With PeakTrace:Box we never see your sequences, and it can run without a network connection.
What if PeakTrace does not suit my facility?
PeakTrace comes with a 60 day, full money back guarantee. Cancel within 60 days for a full refund and just tell us why.