PeakTrace basecaller · Sanger sequencing

Up to 50% more high quality bases.

PeakTrace is a better Sanger sequencing basecaller. Run it online or on your own computer.

Try PeakTrace Online Pro See the evidence

50%

more high quality bases, at most

ways to run PeakTrace

trace parameters reported by QualTrace III

100M+

traces improved

01 · Choose your setup

One basecaller, four ways to use it

PeakTrace Online Pro Runs on: web browser
For: new users trialling the full version PeakTrace for Chromas Runs on: desktop, with Chromas
For: small facilities and end users Auto PeakTrace RP Runs on: Windows and macOS
For: small to medium facilities PeakTrace:Box Runs on: onsite hardware
For: medium to large facilities

02 · The evidence

Same trace. Better call.

In a validation study of 1,643 Sanger traces, PeakTrace increased average aligned Q20+ read length by 19.9% over ABI's KB basecaller (954 vs 795 bases) and predicted the true error rate more accurately.

Validation of PeakTrace

Comparison of ABI KB basecalling (left) with PeakTrace basecalling (right) on the same trace

03 · Quality control

QualTrace III

QualTrace III reports 55 trace parameters covering run conditions and trace quality, so facility managers can spot a problem in a run and find its cause quickly.

Extends capillary array life
Helps optimise sequencing protocols
Gives end users useful feedback on their traces
Tracks the run conditions of every sample

Try QualTrace III online

04 · Log

News and how-tos

10 May 2026In depth look at PeakTrace 6
4 May 2025How to Install PeakTrace License Update
13 May 2024How to Update the PeakTrace USB Key Time
22 Dec 2023Christmas New Year 2023
16 Jun 2023Auto PeakTrace RP 6.961 Released
14 Jun 2023Auto PeakTrace 6.961 Released

Up to 50% more high quality bases.

One basecaller, four ways to use it

Same trace. Better call.

QualTrace III

News and how-tos

Try PeakTrace on your own traces.