01 · Troubleshooting
What does your trace look like?
Showing all 8 common problems
Failed sequencing reactions
Mixed trace signal (multiple peaks)
Short reads or poor quality basecalls
Poorly resolved peaks (blurry peaks)
Excess free dye (dye blobs)
Weak or noisy trace peaks
PCR or primer dimer in the reaction
Sharp signal spikes in the trace
Nine more guides cover slippage, hard stops, chemistry breakdown, indels, chimeras and more.
Full troubleshooting guide02 · Automatic checks
Let QualTrace III find the problem
Checking traces by eye gets slow beyond a handful. QualTrace III scans the raw data for many sequencing problems, so it works with any basecaller. Upload your own traces to the free online version.
03 · Manuals and protocols
Documentation
Current products
Material safety data sheets
Older products
- QualTraceManual
- dLUTE SEQ reagentManual
- UniSeqManual
- UniSelectManual
- UniFinishManual
- CounterTraceManual
- CounterTrace quick startGuide
- CounterTrace phred integrationGuide
- CounterTrace II quick startGuide
Protocols
- Tips for improving DNA sequencing reactionsProtocol
- Exonuclease I / SAP clean-up of PCR productsProtocol
- Reaction clean-up with phenol and butanolProtocol
Software downloads
The PeakTrace RP and PeakTrace:Box download pages are password protected. Email us for the password.