General Information
The DNA Sequencing Center (DNASC) at Brigham Young University was established to help researchers and students process DNA samples efficiently and economically. The DNASC is committed to serving the BYU research community by providing advanced and efficient services for DNA sequencing and analysis.
DNA Sequencing Instruments Used
- ABI 3730xl
- Illumina GAIIx
- 454 Life Sciences GS FLX+
DNA Sequencing Prices
Internal Rates (BYU PIs Only)
- $6.50 – Sequencing Charge per sample (BigDye Rxn, Clean-up + Electrophoresis)
- $0.80 – Lane Charge per sample (Electrophoresis only)
- $692.00 -(Typically enough for 1,600 reactions if used sparingly)
External Rates
- $9.00 – Sequencing Charge (BigDye Rxn, Clean-up + Electrophoresis)
- $1.20 – Lane Charge (Electrophoresis only)
- Costs go down with higher volume
Sequencing Plans – include BigDye reaction, clean-up and electrophoresis. Purified DNA templates and primers need to be provided at the right concentration
| PLAN | SAMPLES | PRICE/SPL | TOTAL COST |
| ES-0 | 1 | $9.00 | $9.00 |
| ES-1 | 100 | $8.68 | $867.60 |
| ES-2 | 200 | $8.35 | $1,670.40 |
| ES-4 | 400 | $7.83 | $3,132.00 |
| ES-6 | 600 | $7.06 | $4,233.60 |
| ES-8 | 800 | $6.41 | $5,126.40 |
| ES-10 | 1,000 | $6.03 | $6,030.00 |
| ES-20 | 2,000 | $5.58 | $11,160.00 |
| ES-40 | 4,000 | $5.40 | $21,600.00 |
| ES-60 | 6,000 | $5.22 | $31,320.00 |
Lane Plans – include only electrophoresis. Users need to perform BigDye reaction and clean-up reactions at their own cost. Samples must be dried before submission.
| LAN | SAMPLES | PRICE/SPL | TOTAL COST |
| EL-0 | 1 | $1.30 | $1.30 |
| EL-1 | 100 | $1.20 | $120.00 |
| EL-2 | 200 | $1.12 | $224.00 |
| EL-3 | 300 | $1.05 | $315.00 |
| EL-4 | 400 | $1.00 | $400.00 |
| EL-6 | 600 | $0.92 | $552.00 |
| EL-8 | 800 | $0.85 | $680.00 |
| EL-10 | 1,000 | $0.82 | $820.00 |
Sample Requirements
Plasmid DNA
- 300 ng
PCR Products
- 3 ng for products under 200 bp
- 10 ng for products under 500 bp
- 20 ng for products under 1,000 bp
- 40 ng for products under 2,000 bp
Primer
- 3.2 pmoles
Other information
Costs are lower with higher volumes
Sequencing Turn Around Times
24 – 48 hours
International orders
No
GLP DNA sequencing
Not Mentioned
Other DNA sequencing related services offered
- Next Generation Sequencing
- Fragment Analysis
- SNP analysis
- Primer walking
Contact Information
DNA Sequencing Center at Brigham Young University
690-A WIDB
Provo, UT 84602
USA
Phone: +1 801 422 3647
Fax: +1 801 422 0090
Staff: Edward Wilcox – Manager – Email: dnasc@byu.edu
Website: http://dnasc.byu.edu/Home.aspx
Comments
Reduced price due to central equipment and expertise with increased efficiency and quality data. Quite cheap for electrophoresis only.
Return to the main DNA sequencing services review page.