Developer: Technelysium Pty Ltd
Supported platforms: Windows 7 SP1, 8, 10, and 11 (stable release: v2.2.0; ChromasPro 3 v2.9.0 Beta also available)
Download: https://technelysium.com.au/wp/chromaspro/
Features
View Quality scores
Yes. Displays base quality scores and uses them automatically for low-quality end trimming and contig assembly.
View raw data
Focuses on aligned processed chromatograms inside contigs rather than batch raw-intensity exports (unlike the free companion Chromas viewer, which includes a raw trace display toggle and intensity export).
Assembly
Yes. De novo and reference-guided sequence assembly supporting Sanger traces (.ab1, .scf, .ztr) as well as 454 and Illumina reads (up to 1,000,000 sequences with 8 GB+ RAM), complete with a graphical contig editor and discrepancy highlighting.
Input file formats
.ab1, .scf, .ztr, .sff, FASTA, FASTQ, EMBL, GenBank, SwissProt, GenPept, GCG RSF, and plain text.
Output file formats
.ab1, .scf, FASTA, FASTQ, printed reports, and clipboard images of chromatograms, ORFs, and restriction maps.
Trimming
Yes. Automated quality-score end trimming plus built-in vector sequence detection and non-destructive trimming.
Integrated Blast
Yes. Direct nucleotide and protein NCBI BLAST submission directly from sequence or contig selections (handling alignment gaps cleanly and bypassing URL length limits).
Open multiple windows
Yes, including simultaneous project, contig alignment, trace, ORF, and restriction map views.
Edit bases
Yes, with live amino-acid translation while editing and ambiguity flagging.
ABI limits (regions outside of clear range region are displayed in gray)
Import and display of instrument ABI clear-range tags is not confirmed; ChromasPro provides its own stored quality and vector trim annotations.
Other functions
- ClustalW multiple sequence alignment integration
- Restriction enzyme mapping, ORF finding with G+C plots, and protein hydrophilicity/antigenicity plots
- Reverse complement of sequences and chromatograms plus exact/optimal sequence search
Cost
Paid Windows software with a 14-day free trial. Academic licences cost $8 USD/month or $149 USD perpetual; commercial licences cost $16 USD/month or $299 USD perpetual (upgrades included).
Comments
ChromasPro is one of the best-value commercial upgrades for Windows users who already know Chromas and simply need multi-read contig assembly, vector removal, and reference-guided proofreading without paying four-figure enterprise suite prices.
Return to the Trace Viewers overview or check trace quality online with QualTrace III Online.