Developer: ConductScience (Scientific File Format Tools)
Supported platforms: Web browser (Windows, macOS, Linux, ChromeOS)
Access: https://conductscience.com/tools/file-formats/ab1-chromatogram
Features
View Quality scores
Yes. Displays per-base Phred quality scores, colour-codes bases by quality tier (highlighting Q20/Q30 benchmarks), and flags ambiguous base calls.
View raw data
No raw capillary trace channel view.
Assembly
No.
Input file formats
.ab1 (ABIF) and .scf via local drag-and-drop, plus a built-in 200 bp GFP demo chromatogram.
Output file formats
FASTA and CSV exports.
Trimming
Suggests 5′ and 3′ quality trim points in the browser.
Integrated Blast
No.
Open multiple windows
Yes, via multiple browser tabs.
Edit bases
Focused on read-only inspection and export rather than chromatogram base editing.
ABI limits (regions outside of clear range region are displayed in gray)
No.
Other functions
- Client-side browser parsing (no file uploads to remote servers and no account required)
- Instant demo chromatogram loading and built-in method notes
Cost
Free (marked for research use only).
Comments
ConductScience Chromatogram Viewer is a clean, privacy-friendly browser tool when you want to check a .ab1 or .scf file locally without uploading proprietary sequences to an external server or registering an account. Its Phred colour-coding, suggested trim bounds, and CSV/FASTA export make it handy for quick lab QC checks.
Return to the Trace Viewers overview or check trace quality online with QualTrace III Online.