Developer: Microcline Projects (Conan K Woods)
Supported platforms: Web browser (Windows, macOS, Linux, ChromeOS; works offline when saved locally)
Access: https://chromatogramviewer.microclineprojects.com/ (project directory: microclineprojects.com)
Features
View Quality scores
Yes. Individual base peaks are colour-coded as good, acceptable, or poor quality, accompanied by summary counts of bases with quality ≥10 and ≥30.
View raw data
Exposes all hidden internal ABIF/SCF metadata fields (well ID, basecaller version, polymer lot number, run protocol, instrument name, voltages, etc.) with one-click clipboard export; the trace graph displays processed chromatogram peaks.
Assembly
No.
Input file formats
.ab1 (ABI) and .scf.
Output file formats
Clipboard export of internal trace metadata fields and sequence text.
Trimming
No automated quality trimming.
Integrated Blast
No.
Open multiple windows
Yes, via multiple browser tabs.
Edit bases
Read-only inspection tool (includes reverse-complement toggle and keyboard arrow base-by-base navigation).
ABI limits (regions outside of clear range region are displayed in gray)
No.
Other functions
- 100% client-side JavaScript / HTML5 Canvas execution—no trace data is uploaded to any server
- Page can be saved locally for air-gapped or offline chromatogram review
- Full internal ABI/SCF tag inspection for troubleshooting sequencer runs
Cost
Free.
Comments
Microcline Chromatogram Viewer stands out among lightweight browser viewers for exposing internal ABIF/SCF header metadata (such as polymer lot, voltage, and well ID) while keeping all parsing strictly client-side. It is especially useful for sequencing facilities and troubleshooting QC without installing desktop software.
Return to the Trace Viewers overview or check trace quality online with QualTrace III Online.