Developer: SeqSMART
Supported platforms: Web browser (Windows, macOS, Linux, ChromeOS)
Access: https://seqsmart.com/sanger
Features
View Quality scores
Visualises peak quality and noisy base regions alongside called variants (aimed at human variant review rather than Phred histogram exporting).
View raw data
Displays coloured chromatogram peaks aligned under reference and expected tracks rather than unprocessed capillary sensor sweeps.
Assembly
Automatic single-read human genomic reference identification and alignment without manual reference selection; does not perform general multi-trace de novo plasmid contig assembly.
Input file formats
.ab1.
Output file formats
In-browser annotated variant tables (coordinates, HGVS cDNA/protein notation, dbSNP, zygosity) and interactive alignment inspection (no dedicated offline trace/alignment file export established).
Trimming
Yes—includes interactive toolbar controls for Trim, Zoom, Viewport, and Reverse View.
Integrated Blast
Uses instant automated human reference sequence lookup rather than general NCBI BLAST against all species.
Open multiple windows
Yes, via multiple browser tabs.
Edit bases
Supports interactive variant curation—analysts can remove false-positive noise calls or click positions in the chromatogram to manually add alleles and assign zygosity.
ABI limits (regions outside of clear range region are displayed in gray)
No.
Other functions
- Dedicated InDel Detector track flagging shifted/overlapping peaks from heterozygous indels
- Multi-track layout comparing Reference Sequence, Original AB1 File, and Expected Sequence
- Integrated genomic context browser beneath the trace showing transcript and known-variant distributions
Cost
The standalone browser-based Sanger Sequencing module is free (sessions do not permanently retain uploaded traces). The broader SeqSMART clinical NGS/VCF platform is commercial quote-based.
Comments
SeqSMART’s Sanger module is convenient for human clinical or Mendelian genetics labs because you can drop in a .ab1 file with zero configuration and watch it auto-locate the human gene reference, annotate HGVS/dbSNP variants, and flag indels. For bacterial cloning, plasmids, or non-human targets, use a general alignment viewer such as GEAR Genomics or Benchling.
Return to the Trace Viewers overview or check trace quality online with QualTrace III Online.