General Information
DNA sequencing and Genomics laboratory is part of the Institute of Biotechnology, an independent research unit of the University of Helsinki. The laboratory was founded in 2008, situated on the Viikki campus in eastern Helsinki that provides DNA sequencing service.
DNA Sequencing Instruments Used
- Applied Biosystems 3130xl
- Applied Biosystems SOLiD 4
- Applied Biosystems SOLiD 5500XL
- Illumina MiSeq
- Roche 454 GS FLX+
- PacBio RS
DNA Sequencing Prices
Prices for one sequencing reaction
- University of Helsinki | 15 Ä
- Other universities in Finland | 20 Ä
- Non-academic institutions | 30 Ä
Prices for sequencing 96-well plate
- University of Helsinki | 550 Ä
- Other universities in Finland | 650 Ä
- Non-academic institutions | 800 Ä
DNA Template Requirements
Plasmids under 10kb
- 5µl at a concentration of 100ng/µl
Cosmids, BACs, PACs
- 500 ng – 1µg
PCR products over 150 bp
- 20 ng/µl
Primers
- 5 pmol/µl
Other information
The facility have several standard primers for the most common vectors and available at no extra cost.
Sequencing turn-around time
3 – 5 days
International orders
Yes
GLP DNA sequencing
Not mentioned
Other DNA sequencing related services offered
- Next Generation Sequencing
- Fragment Analysis
- Quantitative PCR
- Genotyping
- Micro Array Analysis
Contact Information
Petri Auvinen, Laboratory Director
Viikinkaari 4
P.O.Box 56
00014
University of Helsinki
Finland
Phone: +358 (0) 2941 58884 | +358 (0) 2941 58902
Fax: +358 (0) 2491 58952
Email: bio-alf@helsinki.fi | petri.auvinen@helsinki.fi
Website: http://www.biocenter.helsinki.fi/bi/dnagen/index.htm
Comments
Large projects can negotiate about discount (10 % – 15 %) on regular prices. A typical sequencing reaction gives about 650-700 bases of reliable sequence. Sequencing turn-around time is quite long.
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