PeakTrace Basecaller

Basecalling software for obtaining longer reads with fewer errors from the ABI 377, 310, 3700, 3730, 3100, 3130, 3500 & 3730xl DNA sequencers


What is the PeakTrace Basecaller

PeakTrace is a DNA sequencing basecaller that provides lower errors and improve the read length of ABI sequencing traces by up to 50%. The PeakTrace basecaller works with any trace produced by an ABI sequencer including the ABI 377, ABI 310, ABI 3700, ABI 3730, ABI 3730xl, ABI 3100, ABI 3500, or ABI 3130. PeakTrace also supports the MegaBACE DNA sequencers.

comparison of PeakTrace basecalling with ABI KB basecalling

Comparison of the PeakTrace basecalling with ABI basecalling.

Common questions asked about PeakTrace

How is PeakTrace different to other basecaller like KB?

Simply put, PeakTrace is a better basecaller than KB in exactly the same way that KB is a better basecaller than phred or the old ABI basecaller. PeakTrace provides longer reads, more high quality bases, and better looking traces than KB or any other basecaller.

How do I use PeakTrace?

There are four ways to use PeakTrace. The easiest is to use the free online version of PeakTrace which is designed for users with only a need to basecall a few traces or want to see what PeakTrace can offer on their own data. This provides exactly the same quality as the paid services, however, you are limited to processing only 6 traces per day.

If you need to basecall more traces then 6 per day you will need to register for the FTP PeakTrace service or register for Auto PeakTrace RP. After registering you will need to purchase a number of PeakTrace basecalling units from our secure online store, or establish a standing account with us. You will be provided with a ftp account on our server where you can upload your traces to using any ftp program or a link to download the Auto PeakTrace RP software.

The fourth way is to use our standalone PeakTrace Basecaller, the PeakTrace:Box. This is a full hardware/software system that you can install in your facility and run PeakTrace locally without the need to upload your traces to our server. The PeakTrace:Box system is ideal for users with large numbers of traces to process, or for customers with privacy concerns. For more information on the PeakTrace:Box system please go to the PeakTrace:Box overview page.

If you want to visually compare the read length improvements offered by PeakTrace we recommend you use a trace viewing application like ABI's Sequencing Analysis or Chromas.

How much does PeakTrace FTP cost?

The PeakTrace FTP is sold on a per trace basis, however, we do not charge you unless the PeakTrace service improves your trace. The current prices for the PeakTrace FTP basecalling service are:

  100 PeakTrace Basecallings US$30.00
  500 PeakTrace Basecallings US$130.00
  2,000 PeakTrace Basecallings US$480.00
  10,000 PeakTrace Basecallings US$2000.00

How much does Auto PeakTrace RP cost?

The Auto PeakTrace RP software is free, but to use it you need to register to obtain a copy of the software. Once registered, you need purchase processing units from our online store or establish a standing account with us. Auto PeakTrace RP does not have a the improvement guarantee that the FTP service does, but it provides much greater control over the processing parameters. The current prices to purchase Auto PeakTrace RP basecalling units are:

  100 PeakTrace Basecallings US$30.00
  500 PeakTrace Basecallings US$130.00
  2,000 PeakTrace Basecallings US$480.00
  10,000 PeakTrace Basecallings US$2000.00

How much does the PeakTrace:Box system cost?

The monthly lease prices for the PeakTrace:Box systems are:

The three PeakTrace:Mini system (500, 1000, 2000) have an addition once only hardware set up fee of $500. Lease length of less than six month may require a hardware deposit. We also offer significant discounts on the PeakTrace:Box system for long term contracts and/or higher volumes.

If you are processing more than 4000 traces per month then the box system is likely to better suited for your needs. Less than 2000 traces per month and the Auto PeakTrace RP or FTP service will likely be a better fit for your needs.

Which version should I use?

This is a difficult question to answer as it depends on your particular needs and circumstances. Try the free online version initially to see what PeakTrace can do and then please and we will advise you on the best version for your needs.

Can I try PeakTrace for free?

Yes. We offer a free web-based version of PeakTrace which you can try for free. You do not need to register to use this version. The basecalling from the free PeakTrace software is exactly the same as the FTP and PeakTrace:Box systems. The only limitation with the free version is that you are limited to processing only 6 traces per day. If you need to process more than this many traces per day then please contact us.

How do I purchase PeakTrace?

To purchase PeakTrace basecalling units please visit our secure online shop. If expect to be basecalling over 4000 traces per month please as we offer significant discounts for large standing orders.

To purchase the PeakTrace:Box system please .

Will you keep my traces?

No. We have a very strict privacy policy regarding your data (please see our privacy policy for more details). We will never release your data to others, in fact, we will not examine your traces ourselves except with your written permission.

How does PeakTrace work?

PeakTrace is the result of 12 years of R&D at Nucleics into improving DNA sequencing quality. While developing products like the CounterTrace DNA sequencing system, CounterTrace II DNA sequencing system and LongTrace we have learnt a great deal about how to best process and basecall the raw data in DNA sequencing traces. The algorithms that we developed is what allows the PeakTrace basecalling software to extract more usable bases from each trace. For more information about what PeakTrace can do and how it can be used please visit the PeakTrace basecaller overview page.

I want to know more about PeakTrace

Please visit the PeakTrace DNA sequencing overview page or the PeakTrace FAQ page. If you have any further questions please contact our or visit our PeakTrace forum to ask any question you may have.


PeakTrace Whitepaper

Download the
PeakTrace White Paper