General Information
The Genomics lab was established within the Technology facility as part of the unique central support facility for bioscience research. The facilities are not only available to researchers within the University of York but, to academic and industrial researchers from outside the University of York.
DNA Sequencing Instruments Used
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Applied Biosystems 3130xl
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Life Tech Ion Torrent PGM
DNA Sequencing Prices
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Full service | £ 8.71 per reaction | £616.77 per plate
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Run-only service | £ 4.96 per reaction | £352.44 per plate
DNA Template Requirements
Single-stranded
- 15-25 ng/µl
Double-stranded (Plasmid DNA)
- 100-150 ng/µl
Cosmid, BAC
- 0.1-0.5 µg/µl
Bacterial genomic DNA
- 2-3 µg
PCR Product:
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100-200 bp | 1-2 ng/µl
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200-500 bp | 3-5 ng/µl
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500-1000 bp | 5-10 ng/µl
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1000-2000 bp | 10-20 ng/µl
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more than 2000 bp | 15-25 ng/µl
Primers
- 3.2 uM
Other information
Samples, and order forms, need to be submitted by midday Mondays and Thursdays.
Sequencing turn-around time
2 – 3 days
International orders
No information
GLP DNA sequencing
Not mentioned
Other DNA sequencing related services offered
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Next Generation Sequencing
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Genotyping
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Quantitative PCR
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Bioinformatics
Contact Information
Dr Peter Ashton
Technology Facility
Department of Biology (Area 15)
University of York
Wentworth Way
York YO10 5DD
UK
Phone: +44 (0)1904 32 8808 | +44 (0)1904 32 8794
Fax: +44 (0)1904 32 8804
Email: peter.ashton@york.ac.uk
Website: http://www.york.ac.uk/biology/technology-facility/genomics/
Comments
The mentioned prices are for University of York researches, contact facility to know the external prices. Full plate rate will be charged even if there are less than 96 samples on the plate. High Through-put Expressed Sequence Tag (EST) library sequencing is available. Single or both end sequencing from glycerol stocks, colony plates, plasmid preps etc.