General information
The Dana-Farber/Harvard Cancer Center (DF/HCC) DNA sequencing core facility offers large and small-scale DNA sequencing to members of the DF/HCC community as well as to external users.
Sequencing instruments used
Applied Biosystems 3730xl
Prices
Single sample
Full service (external customers) | $6.50
Full service (Dana-Fraber/Harvard University customers) | $5.50
Volume discounts
48+ samples (external customers) | $4.50
48+ samples (Dana-Fraber/Harvard University customers) | $3.50
Other information
Free testing of large project templates and extra discount pricing for
orders over 500 samples. Free DNA mini-preps for orders over 500 samples.
Sample requirements
Plasmids
100 ng/µl | 12 µl minimum
PCR products
5 ng/µl per 100 bp | 12 µl minimum
Primers
1.2 pmol/µl | 12 µl minimum
Common universal primers are provided free.
Sample turn around time
24 hours
International orders
Yes
GLP DNA sequencing
No
Other DNA sequencing related services offered
- Plasmid minipreps for glycerol stocks
- Large scale DNA sequencing
Contact Information
DF/HCC DNA Resource Core
240 Longwood Ave
L.H.R.R.B. Rm212
Boston, MA 02115
(617) 432-7708
email. li_chan@hms.harvard.edu
Comments
Excellent single reaction prices especially for internal customers. Also offer a free re-run of failed sequencing reactions (i.e. same templates and primers) to test for problems originating at the sequencing facility.
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