General Information
MobixLab is a central facility that provides molecular biology expertise and services to the research community, both inside and outside McMaster. Mobixlab provides service and support in Oligo synthesis, DNA sequencing, and fragment analysis.
DNA Sequencing Instruments Used
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Applied Biosystems 3730
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Roche 454 GS FLX+
DNA Sequencing Prices
McMaster Account
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Standard sequencing | $7.50
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Hairpin, AT-rich, Lambda Protocols sequencing | $9.00
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Premixed in 96 well Semi-Skirted plate | $400.00/plate
External Customers
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Standard sequencing | $10.00
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Hairpin, AT-rich, Lambda Protocols sequencing | $14.00
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Premixed in 96 well Semi-Skirted plate | $532.00/plate
DNA Template Requirements
Plasmids
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Up to 10 kb | 100ng/µl
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10 kb-40 kb | 200-500 ng/µl
Bacterial Genomic DNA
- 2000-3000 ng/µl
PCR Product
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100-200 bp | 1-3 ng/µl
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200-500 bp| 2-6 ng/µl
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500-1000 bp| 5-10 ng/µl
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1000-2000 bp| 10-40 ng/µl
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>2000 bp | 40-100 ng/µl
Primers
1 uM
Other information
Bacterial Genomic DNA reactions are more expensive and usually yield 100 to 300 bases of sequence.
Sequencing turn-around time
48 hours
International orders
No information
GLP DNA sequencing
Not mentioned
Other DNA sequencing related services offered
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Next Generation Sequencing
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Fragment Analysis
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Oligosyntheis
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Genotyping
Contact Information
Mobixlab
McMaster University, LSB-B123
1280 Main St. W.
Hamilton, ON
L8S 4K1
CANADA
Phone: +1 905-525-9140
Fax: +1 905-526-1427
Email: mobixlab@mcmaster.ca
Website: http://www.science.mcmaster.ca/mobixlab/
Comments
Standard sequencing reaction usually yields 700-900 bp sequence data. McMaster researchers can pick up printouts of electropherograms and sent to external customers via FedEx courier.