General Information
Molecular Biology Resource Facility (MBRF) in the University of Tennessee campus (more commonly known on the campus as “The Sequencing Lab”) is a self-supporting core facility specializing in the sequencing and analysis.
DNA Sequencing Instruments Used
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Applied Biosystems 3730
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Life Tech Ion Torrent
DNA Sequencing Prices
University
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Full service sequencing | $4.50 per reaction
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Load-Only sequencing | $3.00 per sample
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Discount 96 well tray Load Only | $2.85 per sample
Non-University
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Full service sequencing | $8.00 per reaction
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Load-Only sequencing | $6.00 per sample
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Discount 96 well tray Load Only | $5.00 per sample
DNA Template Requirements
Plasmids
- 750ng/reaction
PCR products
- 15ngs per 100 base pair
Primers
- 10 µM
Other information
All samples sent to the MBRF for Sanger sequencing should be supplied in water, rather than buffer.
Sequencing turn-around time
24 hours
International orders
No information
GLP DNA sequencing
Not mentioned
Other DNA sequencing related services offered
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Next Generation Sequencing
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Library Preparation
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Fragment Analysis
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Genotyping
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Quantitative PCR
Contact Information
Joseph A. May, Manager
Molecular Biology Resource Facility
A211 Walters Life Sciences Bldg
University of Tennessee
Knoxville, TN 37996-0830
USA
Phone: +1 (865) 974-6231
Email: bioseq@utk.edu
Website: http://mbrf.utk.edu/
Comments
The prices are low as the facility operates on cost recovery basis. The facility provides up to 1000 base pair sequences. All services are performed on a first come, first served basis.