General Information
Nucleic Acids Research Facilities (NARF) at Virginia Commonwealth University includes four core laboratories, the Gene Synthesis Core, the DNA Sequencing Core, the Real Time PCR Core, and the MicroArraying Core.
DNA Sequencing Instruments Used
-
Applied Biosystems 3730xl
-
Applied Biosystems 3130xl
DNA Sequencing Prices
Internal
-
Standard sequencing | $6/reaction
-
High Throughput | $4/reaction
External
- Standard sequencing | $9/reaction
DNA Template Requirements
Plasmids
- 100ng/µl
Cosmid, BAC
- 200-500ng/µl
Genomic DNA
- 500-1000ng/µl
PCR products
- 10ng/µl
Primers
- 20ng/µl
Other information
A photograph of 1µl of template sample on agarose gel is preferred. DNA template should be diluted in dH20 not TE buffer. The facility provides free standard primers.
Sequencing turn-around time
2-3 days
International orders
No information
GLP DNA sequencing
Not mentioned
Other DNA sequencing related services offered
-
Fragment Analysis
-
Genotyping
-
Gene Synthesis
-
Quantitative PCR
-
Micro Array Analysis
Contact Information
Nucleic Acids Research Facilities
Virginia Commonwealth University
Room 5050, Sanger Hall
Richmond, Virginia 23298
USA
Phone: +1 (804) 828-6218
Email: dnacore@vcu.edu
Website: http://www.narf.vcu.edu/index.html
Comments
DNA Core Facility provides competitive DNA sequencing. The sequencing is cost effective for internal customers and turnaround time is same day or 24 hours. If DNA sequencing fails the facility will assist for troubleshooting of the problem; however, extra fee might be charged.