PeakTrace 6 allows users to trim traces based on 5 parameters, Q average, N count, Q count, PCR base and Clear Range trimming. These settings allow the removal of unwanted low quality data, particularly on the 3' end. The trim improved traces … [Read more]
Auto PeakTrace RP 6.93 Released
We have released Auto PeakTrace RP 6.93. This release improves the calling of Auto PeakTrace RP from the command line. The full list of changes includes New Feature. Command line control of the proxy server settings. New feature. Custom … [Read more]
Auto PeakTrace 6.931 Released
We have released Auto PeakTrace 6.931 and PeakTrace.exe 6.921 for Windows. This minor update fixes two issue that can cause PeakTrace to crash on rare occasions. These bugs fixes have been made possible by customers sending us example .ab1 files … [Read more]
In Depth: Mixed Peak Threshold over 100%
One of the less obvious setting in PeakTrace is using a mixed peak threshold value over 100%. The mixed peak threshold controls the value at which as basecall with a secondary peak is called as a mixed base. For example, if the mixed peak threshold … [Read more]
Auto PeakTrace 6.93 Released
We have released Auto PeakTrace 6.93. This update improves the control of Auto PeakTrace from the command line and brings it into parity with the peaktrace.exe command line version. It is recommend that all users of peaktrace.exe change to using Auto … [Read more]
In Depth: Clear Range Trimming
The Clear Range Trim option allows you to select the number of bases you want to trim from the start and end of a trace. Through the use of 2 value boxes users can choose how many bases they want to trim from the 5' end of a trace and how long the … [Read more]
In Depth: Fuse Trace and Fuse Basecall
The PeakTrace 6 fuse trace at base and fuse basecall at base options allow the combination of 2 basecallers in one basecall to gain the best from both. Fuse Trace at Base The fuse trace at base option allows you to enter an approximate position … [Read more]
Christmas New Years Holiday at Nucleics 2018
What a great year 2018 has been! Nucleics will be shutting down for our annual company holiday (vacation) break over the Christmas/New Year period and all relaxing at the beach. We will be closed from the 22nd December 2018 until 7th January 2019. I … [Read more]
In Depth: Skipping Short Traces
The skip short traces option is a useful option in PeakTrace to skip over the reads form short DNA fragments. As the improvement in basecall quality for shorter traces can be minimal, you may wish to skip over them and save units. Using this setting … [Read more]
In Depth: No Peak Resolution
An unfortunate side effect of a basecallers ability to process peaks is that the processing can suppress or raise the secondary peak signal and with it alter the recognition of polymorphic sites. The PeakTrace no peak resolution setting outputs … [Read more]
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