I would like to know if anyone has ever tried DNA extraction from excrements using QIAamp with Buffer ASL and Buffer AL that was previously incubated at 70°C. Were results significantly different when using these buffers without incubating although they presented precipitates?
Anything with a lot of humic acids in it hard to do. My advice for those reading this question is use the protocol I published in collaboration with Ed Philips lab.
Improved methods for the isolation of cyanobacterial dna from environmental samples
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