Gene polymorphism

[LLL]

Can anyone help me w/my assignment? I’ve been given part of a gene (240bp; 301-541) with a polymorphism “y” at 405.I must design an assay and describe it in detail (controls, reagents, probes/primers, etc). Any info would be helpful. LL

[FunctionalBio]

Hi, i work for a sequencing service called Functional Biosciences, Inc. We offer SNP resequencing, so I know how to do this if you’re going to go with sequencing rather than probes etc.

Just do a PCR reaction, making sure to run negative controls alongside your DNA samples. Do an exo sap clean up of the pcr reaction. Sequence from both directions using ABI big dye v 3.1, just use the pcr primers if the band on the gel looks clean. Theres a program called Mutation Surveyor that will analyze the traces at the polymorphic site. It can objectively designate each sample as a heterozygote or either type of homozygote. We have this software at our lab, its decent. Otherwise i think theres some free snp software here, [snp.wustl.edu]
but i havent used any of it.

[Author]

Posts