We have upgrade the PeakTrace basecalling of ABI 377 traces. The results are rather astounding (even for me) – we even have one 377 trace which gives over 1000 Q20+ bases!
At this stage support of ABI 377 traces is limited to the BigDyeV3.1 chemistry. If you are using BigDye v1.1 please contact us as the reason for our inability to support BigDyev1.1 is we do not have any example ABI 377 traces using this chemistry to train the software.
I just noticed this message. I have one customer who we have done a lot of sequencing for who uses Big DYE V1.1. If you need data I could contact them and ask if they would be willing to let you use their data for testing. Let me know.
That would be great. I will email you directly about this as I really would like to add BigDye 1.1 support to PeakTrace.
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