problems – sequencing with reverse primer

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- October 12, 2013 at 7:07 am #1282
  
  Anonymous
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  I’m getting problems to sequence yeast DNA (Malassezia). I do the sequencing with forward and reverse primers for ITS region and D1/D2 -28S of ribosomal DNA. But, the sequencing with reverse primers for those 2 regions is not working well. The eletropherograms are too noisy. Only the sequences with forward primers are good. Could you help me to solve this problem?
  Thanks.
  
  Angela
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