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sequencing problem

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    • January 4, 2008 at 9:08 am #1308
      Anonymous
      Inactive

      Recently, we upgraded our Applied Biosystems (ABI)3100 DNA Sequencer to an ABI3130XL system. We are sequencing with 50cm capillaries and POP7 polymer.

      When running sequencing reactions (BigDye v3.1), we generate profiles with following characteristic: at the end of the sequence we often registrate an A-peak with (very) strong intensity >> the more when sequence length is shorter. This problem is observed with XTerminator and other purification technologies.

      Do you have any explanation for this?

    • January 4, 2008 at 9:15 am #1309
      Daniel
      Keymaster

      It sounds like it might be a signal spike caused by an air bubble in the capillary. Take a look at this page https://www.nucleics.com/DNA_sequencing_support/DNA-sequencing-sharp-spikes.html

      If you send me the trace I will take a look at it and let you know.

      Cheers

      Daniel Tillett
      Nucleics Support

    • January 4, 2008 at 9:25 am #1310
      Anonymous
      Inactive

      Thanks for your quick reply. This problem always occurs at the end of the sequence. A profile has been sent by email.
      Regards,

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