Sequncing result for agrobacterium plasmid

[Jason]

Hi,

Previously I cloned my sequence into agrobacterium plasmid as later on I need to use it to infect my plants. But when I extracted the agrobacterium plasmid and sent it for sequencing, the result was terrible. The technician told me it was due to the RNA contamination. So, the second time I used an extraction kit to extract the plasmid and send it for second time sequencing. But this time, the result is worse. Can anyone help me? I can send you the sequencing for viewing if you want to. Thanks.

[Daniel]

Sequencing of low copy plasmids can be difficult. What I would recommend is PCR amplifying the region you want to sequence and then sequence the PCR product directly.

Daniel Tillett
Nucleics Support

[Author]

Posts