We have finally updated the Linux Release of PeakTrace to version 5.62 (yes it has been a long time). Due to changes in the CodeMeter runtime support we have had to create two versions of PeakTrace – one for REHL 5 (CentOS 5.11+) and another for REHL 6 (CentOS 6.6+).
The two packages are available on Nucleics Download page. It is highly recommended that all linux users upgrade to this version.
Here is the complete change list for this version.
- Improved basecalling of rapid run traces.
- Improved basecalling of mixed template traces.
- Improved handling of failed traces.
- Updated system requirements.
- Bug fix for basecall alignment for short skipped traces when using trace trim.
- New Feature. Failed traces are now not charged. The exceptions are:
- Traces below the good base improvement threshold where the good base improvement threshold is set to a value higher than -10.
- Traces that can’t be output because the output folder is unwritable.
- New Feature. Faster basecalling and processing. PeakTrace processing is approximately 120% faster than 5.51.
- New Feature. Support added for the following run conditions:
- ABI 3730 Low Voltage POP7 BigDye 3.1
- ABI Limits are only updated for successful traces
- Improved basecalling and trace trimming.
- New Feature. A nolog option to suppress output of the PeakTrace logfile (command line only).
- Improved fuse basecalling option.
- Improved basecalling through large dye peaks.
- A short trace is now considered to be a trace with fewer than 500 bases.
- Improved support for ET terminator traces.
- Improved support for ABI3500 BigDye 1.1 traces.
- New error code 36 added – Input and output paths are the same.
- New error code 38 added – Skipped short trace.
- Good quality threshold and N base threshold now have maximum values of 99.
- The usual bug fixes and tweaks.