Nucleics

Nucleics

Auto PeakTrace 6.932 & PeakTrace 6.932 Released

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Auto PeakTrace 6.932 Options

Auto PeakTrace 6.932 Options

We have released Auto PeakTrace 6.932 and PeakTrace 6.932 for Linux and Windows. This is a small bug fix release that resolves issues on rare trace types, especially short PCR reads. We would like to thank those of our customers who sent us examples .ab1 files containing potential issues. Our policy is to never have known bugs in the latest release and once we are made aware of an issue we aim to rectify it as soon as possible (usually within weeks). If you notice any trace not being processed and basecalled as you expect, or any other issue of concern, then please get in contact with us.

For users of PeakTrace RP the changes have been automatically made on the remote server side so there is no need to upgrade.

PeakTrace:Box users can download the latest update from the PeakTrace:Box Downloads page. If you do not know the password to access the PeakTrace:Box Downloads page please contact us @support@nucleics.com or contact your local supplier for the update.

Auto PeakTrace RP 6.93 Released

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Auto PeakTrace RP 6.93 Overview

Auto PeakTrace RP 6.93 Overview

We have released Auto PeakTrace RP 6.93. This release improves the calling of Auto PeakTrace RP from the command line. The full list of changes includes

  • New Feature. Command line control of the proxy server settings.
  • New feature. Custom PeakTrace log file names and paths can be set from the command line.
  • New feature. Auto PeakTrace RP can be run in watch mode checking for new .ab1 file in input folder from the command line.
  • New feature. Control of message output to the command line window via -sl option switch.
  • New Feature. Header line can be removed from log files with -sh option on the command line.
  • New Feature. Support added for ABI3130 80cm array POP7 runs.
  • If Auto PeakTrace RP crashes in command line mode it will restart in command line mode, but not output any message to the command window.
  • Bug fixes.

Auto PeakTrace RP 6.93 can be downloaded from the PeakTrace RP Downloads page. If you do not know the password to access the PeakTrace RP Downloads page please contact us or your local supplier for the update. The MacOS X version will be released in the coming days along with the automatic update function.

Auto PeakTrace 6.931 Released

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We have released Auto PeakTrace 6.931 and PeakTrace.exe 6.921 for Windows. This minor update fixes two issue that can cause PeakTrace to crash on rare occasions.

These bugs fixes have been made possible by customers sending us example .ab1 files that have caused issues. If you experience any issues please send us example trace(s) as tracking down bugs that cause a crash once every 100,000 traces is much easier for us if we have an example file. Our aim is to always have zero known bugs in all new releases.

This update is a recommend upgrade for all users PeakTrace customers since the bugs that have were fixed have been present for more than 12 months.

Auto PeakTrace 6.931 can be downloaded from the PeakTrace:Box Downloads page. If you do not know the password to access the PeakTrace:Box Downloads page please contact us @support@nucleics.com or contact your local supplier for the update.

Auto PeakTrace 6.93 Released

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Auto PeakTrace 6.93 Options

Auto PeakTrace 6.93 Options

We have released Auto PeakTrace 6.93. This update improves the control of Auto PeakTrace from the command line and brings it into parity with the peaktrace.exe command line version. It is recommend that all users of peaktrace.exe change to using Auto PeakTrace as peaktrace.exe will not be updated in the next major release of PeakTrace. The full list of changes includes.

  • New Feature. Command line control of QualTrace III options.
  • New Feature. Command line control of the manual override settings.
  • New Feature. Command line control of the proxy server settings.
  • New Feature. Custom PeakTrace and QualTrace log file names and paths can be set from the command line.
  • New Feature. Auto PeakTrace can be run in watch mode checking for new .ab1 file in input folder from the command line.
  • New Feature. Control of message output to the command line window via -sl option switch.
  • New Feature. The header line can be removed from log files with -sh option on the command line.
  • New Feature. Support added for ABI3130 80cm array POP7 runs.
  • If Auto PeakTrace crashes in command line mode it will restart in command line mode, but not output any message to the command window.
  • CodeMeter runtime requirement upgraded to 6.80.
  • Minor bug fixes

Auto PeakTrace 6.93 can be downloaded from the PeakTrace:Box Downloads page. If you do not know the password to access the PeakTrace:Box Downloads page please contact us @support@nucleics.com or contact your local supplier for the update.

Christmas New Years Holiday at Nucleics 2018

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What a great year 2018 has been! Nucleics will be shutting down for our annual company holiday (vacation) break over the Christmas/New Year period and all relaxing at the beach. We will be closed from the 22nd December 2018 until 7th January 2019. I (Daniel) will check my emails occasionally over this time, but it might take us longer than usual to get back to you.

PeakTrace 6.92 Command line Versions Released

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Comparison between the KB & PeakTrace Basecaller

Comparison between the KB & PeakTrace Basecaller

We have released the PeakTrace 6.92 command line versions for Windows and Linux. The full list of changes include

  • New Feature. Calculation of the signal start peak location directly from the trace.
  • New Feature. Controlled removal of minor peaks without mixed basecalling.
  • Support for additional run conditions including ultra low voltage runs.
  • ABI 377 support added.
  • Improved basecalling.
  • Improved basecalling of PCR product traces.
  • Improved mixed basecalling.
  • Improved processing of traces with polymorphic peaks.
  • Improved processing of traces with long single base runs.
  • Improved trace trimming.
  • Improved removal of air bubble spikes in the trace.
  • Improved clean baseline and extra baseline processing.
  • CodeMeter runtime requirement upgraded to 6.70a.

PeakTrace 6.92 can be downloaded from the PeakTrace:Box Downloads page. If you do not know the password to access the PeakTrace:Box Downloads page please contact us @support@nucleics.com or contact your local supplier for the update.

If you are using PeakTrace on windows it is highly recommended that users transition to using the command line feature of Auto PeakTrace 6.92.

Auto PeakTrace 6.92 Released

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Auto PeakTrace 6.92 Overview

Auto PeakTrace 6.92 Overview

We have released Auto PeakTrace 6.92. This update supports a new method of controlling the removal of minor peaks from under major peaks as well of hundreds of small changes that add up to a major improvement to trace appearance and basecalling. The full list of changes includes.

  • New Feature. Calculation of the signal start peak location directly from the trace.
  • New Feature. Controlled removal of minor peaks without mixed basecalling.
  • Support for additional run conditions including ultra low voltage runs.
  • ABI 377 support added.
  • Improved basecalling.
  • Improved basecalling of PCR product traces.
  • Improved mixed basecalling.
  • Improved processing of traces with polymorphic peaks.
  • Improved processing of traces with long single base runs.
  • Improved trace trimming.
  • Improved removal of air bubble spikes in the trace.
  • Improved clean baseline and extra baseline processing.
  • CodeMeter runtime requirement upgraded to 6.70a.

Auto PeakTrace 6.92 can be downloaded from the PeakTrace:Box Downloads page. If you do not know the password to access the PeakTrace:Box Downloads page please contact us @support@nucleics.com or contact your local supplier for the update.

The command line version of PeakTrace for Windows and Linux will be released in the coming days, however, it is highly recommended that all Windows command line users transition to using the command line feature of Auto PeakTrace 6.92.

PeakTrace 6.92 Released

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KB PeakTrace Comparison

We have released PeakTrace 6.92. This is a bug fix and enhancement release and is the culmination of six months hard work by the team at Nucleics. We have gone through nearly every line of code within PeakTrace to improve and enhance the processing and basecalling of Sanger sequencing traces. While there is no single massive changes from all this work, the cumulative effect of all these small changes adds up to a major improvement. The full list of changes includes.

  • Support for additional run conditions including ultra low voltage runs.
  • ABI 377 support.
  • Improved basecalling.
  • Improved basecalling and processing of PCR product traces.
  • Improved mixed basecalling.
  • Improved processing of traces with polymorphic peaks.
  • Improved processing of traces with long single base runs.
  • Improved trace trimming.
  • Improved removal of air bubble spikes in the trace.
  • Improved clean baseline and extra baseline processing.

PeakTrace 6.92 is live on our servers so our PeakTrace RP users will be automatically getting the benefits of 6.92. Over the coming week we will be releasing updates for the standalone PeakTrace:Box for Linux and Windows, along with an update for the Auto PeakTrace RP client program for Windows and MacOSX. We will make further announcements when these updates are released.

Updated Nucleics Website

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We have updated the Nucleics website to make it easier to navigate and find information. This will be an work in progress over the coming months so there should be plenty of changes to look forward to.

Support for purchasing PeakTrace in local currencies

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We have recently added support for buying PeakTrace RP units in your local currency with local VAT/GST/Sales taxes include via paddle.com. The list of currencies that can be used includes: Euro (EUR), British Pound (GBP), Chinese Yuan (CNY), Polish Zloty (PLN), Swedish Krona (SEK), South African Rand (ZAR), Swiss Franc (CHF), Brazilian Real (BRL), Ruble (RUB), Australian Dollar (AUD), Canadian Dollar (CAD), Hong Kong Dollar (HKD), Singapore Dollar (SGD), New Zealand Dollar (NZD), Danish Krone (DKK), Hungarian Furint (HUF), Czech Koruna (CZK), Indian Rupee (INR).

While this new way of buying PeakTrace RP units can be advantageous in some situations, the downside is the cost is greater due to the extra fees charged by Paddle. The major use case is for customers located in India who can now use a local Indian credit card to buy PeakTrace RP units without the need for an international credit card. For most other customers the current US$ payment system (handled by Paypal) is likely be best option.