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Auto PeakTrace 6.95 & PeakTrace 6.95 Released

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PeakTrace 6.95 Overview

PeakTrace 6.95 Overview

We have released Auto PeakTrace 6.95 and PeakTrace 6.95 for Windows & Linux. This is a major release that improves PeakTrace and is recommended for all users of the 6.94 or earlier series of PeakTrace. The server backend for Auto PeakTrace RP has also been updated to the latest release of PeakTrace.

The full list of changes.

  • New Feature. Keep bubble spikes in the processed trace using the -ks option.
  • New Feature. Auto PeakTrace 6 will check if you wish to save changes when exiting options window without manually saving.
  • Improved mixed basecalling.
  • Improved crosstalk correction on rare trace types.
  • Improved processing and basecalling of traces with bubble spikes.
  • Improved early basecalling.
  • Bug fixes.

PeakTrace:Box customers can download the latest update from the PeakTrace:Box Downloads page. If you do not know the password to access the PeakTrace:Box Downloads page please contact us at support@nucleics.com or contact your local supplier for the update.

Auto PeakTrace 6.94 & PeakTrace 6.94 Released

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Auto PeakTrace 6.94 Overview

Auto PeakTrace 6.94 Overview

We have released Auto PeakTrace 6.94 and PeakTrace 6.94 for Windows & Linux. This is a major release that improves PeakTrace and is recommended for all users of the 6.93 or earlier series of PeakTrace. The full list of changes.

  • New feature. ABI377 and ABI310 .ab1 files are now output in .ab1 format with quality scores.
  • New feature. Trace files without basecall or processed trace data can be output in .ab1 format using force processing setting.
  • New feature. Support added for the following run conditions and instruments:
    • ABI310
      • 30cm POP4 BigDye 3 & 1
      • 30cm POP6 BigDye 3 & 1
      • 36cm POP4 BigDye 1
      • 50cm POP6 BigDye 3 & 1 Rapid Run
    • ABI3700
      • POP6 BigDye 3 & 1
      • POP5 BigDye 3 o ABI3130/3730
      • 50cm POP7 ET terminator
      • 50cm POP6 ET terminator
    • ABI3130
      • 80cm POP4 BigDye 3 & 1
    • ABI3200
      • 9kV BigDye 1
      • 4kV BigDye 1
    • MJResearch Biostation
  • Default value for q average trim changed to 9 in a window of 40 bases.
  • Default value for good base improvement changed to -10.
  • Error code 25 description changed from “normalization failed” to “overload trace” to make cause of this result clearer.
  • Improved mixed basecalling.
  • Improved basecalling through “Dye Blob” regions.
  • Improved processing of PCR product traces.
  • Bug fixes.

PeakTrace:Box customers can download the latest update from the PeakTrace:Box Downloads page. If you do not know the password to access the PeakTrace:Box Downloads page please contact us @support@nucleics.com or contact your local supplier for the update.

Auto PeakTrace RP 6.94 Released

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Auto PeakTrace RP 6.94

Auto PeakTrace RP 6.94

We have release Auto PeakTrace 6.94 for Windows & MacOSX (Mojave or earlier). This update incorporates a large number of changes and enhancements.

  • New feature. ABI377 and ABI310 .ab1 files are now output in .ab1 format with quality scores.
  • New feature. Trace files without basecall or processed trace data can be output in .ab1 format using force processing setting.
  • New feature. Support added for the following run conditions and instruments:
    • ABI310
      • 30cm POP4 BigDye 3 & 1
      • 30cm POP6 BigDye 3 & 1
      • 36cm POP4 BigDye 1
      • 50cm POP6 BigDye 3 & 1 Rapid Run
    • ABI3700
      • POP6 BigDye 3 & 1
      • POP5 BigDye 3 o ABI3130/3730
      • 50cm POP7 ET terminator
      • 50cm POP6 ET terminator
    • ABI3130
      • 80cm POP4 BigDye 3 & 1
    • ABI3200
      • 9kV BigDye 1
      • 4kV BigDye 1
    • MJResearch Biostation
  • Default value for q average trim changed to 9 in a window of 40 bases.
  • Default value for good base improvement changed to -10.
  • Error code 25 description changed from “normalization failed” to “overload trace” to make cause of this result clearer.
  • Improved mixed basecalling.
  • Improved basecalling through “Dye Blob” regions.
  • Improved processing of PCR product traces.
  • Bug fixes.

Auto PeakTrace RP 6.94 can be downloaded from the PeakTrace RP Downloads page. If you do not know the password to access the PeakTrace RP Downloads page please contact us or your local supplier for the update.

How to update the CodeMeter Runtime

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All version of Auto PeakTrace above 5.70 require the latest CodeMeter Runtime (6.90 as of September 2019). To update you first need to check which version of the CodeMeter Runtime you have installed on your computer. To do this:

1. Open the CodeMeter Control Center from Run > All Programs > CodeMeter. Please make sure that the USB Key supplied by Nucleics is plugged into your computer.

About

2. Click on the Help menu and then on About.

about_runtime

3. The version number will be shown on the About window. If you runtime version is less than 6.80 then you need to update the CodeMeter Runtime. The latest CodeMeter runtime can be downloaded from Wibu. If you are running PeakTrace on Windows XP you will need to use CodeMeter 6.80. This version can be downloaded from the PeakTrace:Box downloads page.

4. Run the Codemeter Runtime Installer following the prompts. After it finishes restart your computer.

Auto PeakTrace 6.936 & PeakTrace 6.936 Released

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Auto PeakTrace 6.936 Overview

Auto PeakTrace 6.936 Overview

We have released Auto PeakTrace 6.936 and PeakTrace 6.936 for Windows & Linux. This is a minor release that squashes a few bugs that have been uncovered over the last couple of months. This update is recommended for all users of the 6.93 series of PeakTrace.

The Auto PeakTrace RP and PeakTrace for Chromas changes are all on the server side so there will not be a corresponding update for these programs at this stage.

PeakTrace:Box customers can download the latest update from the PeakTrace:Box Downloads page. If you do not know the password to access the PeakTrace:Box Downloads page please contact us @support@nucleics.com or contact your local supplier for the update.

Auto PeakTrace 6.935 & PeakTrace 6.935 Released

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Auto PeakTrace 6.935 Overview

We have released Auto PeakTrace 6.935 and PeakTrace 6.935 for Windows & Linux. This is a minor release that improves the basecalling why large or late air bubble spikes are present in the raw data. This update is optional, but if you have noticed issues with extra bases being called in traces with large bubble spikes then you may wish to upgrade.

The Auto PeakTrace RP and PeakTrace for Chromas changes are all on the server side so there will not be a corresponding update for these programs.

PeakTrace:Box customers can download the latest update from the PeakTrace:Box Downloads page. If you do not know the password to access the PeakTrace:Box Downloads page please contact us @support@nucleics.com or contact your local supplier for the update.

Auto PeakTrace 6.934 & PeakTrace 6.934 Released

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Auto PeakTrace 6.934

Auto PeakTrace 6.934 Overview

We have released Auto PeakTrace 6.934 and PeakTrace 6.934 for Windows & Linux. This is a small bug fix release that corrects an issue with the simple mixed basecall as well as a rare thread race issue when archive traces and auto start are both selected. Unless you are using simple mixed basecall (nobody should be as it is inferior to standard mixed basecalling), this update is optional.

The Auto PeakTrace RP and PeakTrace for Chromas changes are all on the server side so there will not be a corresponding update for these programs.

PeakTrace:Box customers can download the latest update from the PeakTrace:Box Downloads page. If you do not know the password to access the PeakTrace:Box Downloads page please contact us @support@nucleics.com or contact your local supplier for the update.

Auto PeakTrace 6.933 & PeakTrace 6.933 Released

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Auto PeakTrace 6.933 Overview

Auto PeakTrace 6.933 Overview

We have released Auto PeakTrace 6.933 and PeakTrace 6.933 for Windows. This is a small bug fix release that fixes rare issues identified by some of our customers. If you are not experiencing any issues with your traces then this update is optional. We aim to always have no known bugs in the latest versions of PeakTrace and will release an update as soon as any issues are identified and fixed.

The Linux version will be released in the coming days and the improvements will be incorporated automatically into the Auto PeakTrace RP and PeakTrace for Chromas versions as the changes are all on the server side.

PeakTrace:Box users can download the latest update from the PeakTrace:Box Downloads page. If you do not know the password to access the PeakTrace:Box Downloads page please contact us @support@nucleics.com or contact your local supplier for the update.

Auto PeakTrace 6.932 & PeakTrace 6.932 Released

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Auto PeakTrace 6.932 Options

Auto PeakTrace 6.932 Options

We have released Auto PeakTrace 6.932 and PeakTrace 6.932 for Linux and Windows. This is a small bug fix release that resolves issues on rare trace types, especially short PCR reads. We would like to thank those of our customers who sent us examples .ab1 files containing potential issues. Our policy is to never have known bugs in the latest release and once we are made aware of an issue we aim to rectify it as soon as possible (usually within weeks). If you notice any trace not being processed and basecalled as you expect, or any other issue of concern, then please get in contact with us.

For users of PeakTrace RP the changes have been automatically made on the remote server side so there is no need to upgrade.

PeakTrace:Box users can download the latest update from the PeakTrace:Box Downloads page. If you do not know the password to access the PeakTrace:Box Downloads page please contact us @support@nucleics.com or contact your local supplier for the update.

Auto PeakTrace RP 6.93 Released

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Auto PeakTrace RP 6.93 Overview

Auto PeakTrace RP 6.93 Overview

We have released Auto PeakTrace RP 6.93. This release improves the calling of Auto PeakTrace RP from the command line. The full list of changes includes

  • New Feature. Command line control of the proxy server settings.
  • New feature. Custom PeakTrace log file names and paths can be set from the command line.
  • New feature. Auto PeakTrace RP can be run in watch mode checking for new .ab1 file in input folder from the command line.
  • New feature. Control of message output to the command line window via -sl option switch.
  • New Feature. Header line can be removed from log files with -sh option on the command line.
  • New Feature. Support added for ABI3130 80cm array POP7 runs.
  • If Auto PeakTrace RP crashes in command line mode it will restart in command line mode, but not output any message to the command window.
  • Bug fixes.

Auto PeakTrace RP 6.93 can be downloaded from the PeakTrace RP Downloads page. If you do not know the password to access the PeakTrace RP Downloads page please contact us or your local supplier for the update. The MacOS X version will be released in the coming days along with the automatic update function.