In Depth

10 May 2026
In depth look at PeakTrace 6
PeakTrace 6 (Auto PeakTrace 6, Auto PeakTrace 6 RP and the Linux PeakTrace 6) has a number of very powerful options that may not have been explored by many users of…
In depthNews
23 May 2023
In Depth: .poly file output
One of the new features of PeakTrace 6.96 is the ability to generate PHRED .poly files. The .poly file has the name [filename].poly where [filename] is the name…
In depthReleasesNews
14 May 2023
Why use PeakTrace?
A question we get asked frequently is why should a core facility or sequencing service company use the PeakTrace Basecaller™ -- after all the KB Basecaller™ works well and is…
In depthNews
24 Sep 2019
In Depth: N Base Trim
The N Base Trim option allows users to trim the 3' or 5' end of a trace based on the number of N base calls in a selected window…
In depth
13 May 2019
In Depth: Q Count Trim
The q count trim option allows end trimming of the low quality regions of the basecall using a count of q score below a set threshold using a…
In depth
1 May 2019
In Depth: Auto Start
Under the default settings, Auto PeakTrace 6 will only start to process trace files after the user has manually clicked the Run button on the main window. If…
In depth
26 Apr 2019
In Depth: Archive Traces
A problem that may be encountered when using Auto PeakTrace™, is reprocessing traces that have not been moved from the input folder since PeakTrace was restarted. This can…
In depth
1 Mar 2019
In Depth: Trim Improved Traces Only
PeakTrace 6 allows users to trim traces based on 5 parameters, Q average, N count, Q count, PCR base and Clear Range trimming. These settings allow the removal of…
In depth
25 Jan 2019
In Depth: Mixed Peak Threshold over 100%
One of the less obvious settings in PeakTrace is using a mixed peak threshold value over 100%. The mixed peak threshold controls the value at which a basecall with…
How-tosIn depth
8 Jan 2019
In Depth: Clear Range Trimming
The Clear Range Trim option allows you to select the number of bases you want to trim from the start and end of a trace. Through the use of…
In depth